hnRNP K (Phospho-Ser284) Rabbit Polyclonal Antibody
Référence A1042-100
Conditionnement : 100ul
Marque : Assay Biotechnology
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Catalog No : A1042
- ReactivityHuman,Mouse,Rat,Monkey
- ApplicationsWB,IHC-p,IF(paraffin section),ELISA
- ModificationPhospho Specific
- SourcePolyclonal Rabbit
- Storage and Stability-20°C/1 year
- Size50 μl
- /Quantity
- Quantity
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Details
- Product NamehnRNP K (Phospho-Ser284) Antibody
- DescriptionThis gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the Cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have
- Catalog NoA1042
- ReactivityHuman, Mouse, Rat, Monkey
- ApplicationsWB, IHC-p, IF(paraffin section), ELISA
- ModificationPhospho Specific
- SourcePolyclonal Rabbit
- DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
- PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration1 mg/ml
- Storage and Stability-20°C/1 year
- Other NameHNRNPK; HNRPK; Heterogeneous nuclear ribonucleoprotein K; hnRNP K; Transformation up-regulated nuclear protein; TUNP
- Molecular Weight (Da)50976
- Gene NameHNRNPK
- Protein NameHeterogeneous nuclear ribonucleoprotein K
- Human Gene ID3190
- Human Swiss Prot No.P61978
- ImmunogenThe antiserum was produced against synthesized peptide derived from human hnRNP K around the phosphorylation site of Ser284. AA range:250-299
- SpecificityPhospho-hnRNP K (S284) Polyclonal Antibody detects endogenous levels of hnRNP K protein only when phosphorylated at S284.
- FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 30', using hnRNP K (Phospho-Ser284) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western Blot analysis of 3T3 cells using Phospho-hnRNP K (S284) Polyclonal Antibody