Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie
 
> 2'-Fucosyllactose [41263-94-9]

2'-Fucosyllactose [41263-94-9]

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Katalog-Nummer HY-N9965-5mg

Size : 5mg

Marke : MedChemExpress


Beschreibung

2'-Fucosyllactose (2'-FL) is an oligosaccharide that could be derived from human milk. 2'-Fucosyllactose regulates the expression of CD14, alleviates colitis and regulates the gut microbiome. 2'-Fucosyllactose stimulates T cells to increase IFN-γ production and decreases IL-6, IL-17, and TNF-α production of cytokines[1][2].

IC50 & Target

Target: IFN-γ, IL-6, IL-17, and TNF-α[1]
In Vitro

2'-Fucosyllactose (2'-FL; 0-12 mg/mL; 48 h) suppress cell-associated CD14 expression and to attenuate LPS (100 μg/mL) stimulated IL-8 secretion in T84 cells[1].
2'-Fucosyllactose (2 mg/mL; 48 h; T84 and HCT8 cells) ameliorates inflammation induced by bacterial invasion. 2'-Fucosyllactose inhibits ETEC invasion and attenuated the consequent IL-8 secretion[1].
2'-Fucosyllactose (2 mg/mL; 48 h; T84 and HCT8 cells) induces macrophage migration inhibitory factor signal pathways that suppress inflammation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2'-Fucosyllactose Related Antibodies

Western Blot Analysis[1]

Cell Line: T84 cells
Concentration: 0, 2, and 4 mg/mL
Incubation Time: 48 hours
Result: Suppressed CD14 mRNA and reduced cell-associated CD14 protein expression.

Western Blot Analysis[1]

Cell Line: T84 and HCT8 cellsT84 and HCT8 cells
Concentration: 2 mg/mL
Incubation Time: 48 hours
Result: Suppressed CD14 mRNA and reduced cell-associated CD14 protein expression.
Inhibited CD14 and NF-κB induction.
Induced iκB and SOCS2 expression and STAT3 phosphorylation.
In Vivo

2'-Fucosyllactose (2'-FL; 100 mg (200 μL); i.g.; daily, for 4 d; C57BL/6 mice with AIEC infection) inhibits AIEC infection and inflammation in vivo[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice (8 weeks) with AIEC (uropathogenic E. coli, and adherent-invasive E. coli) infection[1]
Dosage: 100 mg (200 μL)
Administration: Oral gavage; daily, for 4 days
Result: Had colons lengths were closer to normal.
Inhibited the colonisation of the colonic mucosa by O83-positive bacteria.
Decreased CD14 expression, CD14 mRNA levels, IL-6, IL-17 and TNF-α levels in colonic.
Klinische Studie
Molekulargewicht

488.44

Formel

C18H32O15
CAS. Nr.
Appearance

Solid

Color

White to off-white

SMILES

O=C[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@H]1[C@@H]([C@H]([C@H]([C@H](O1)CO)O)O)O[C@H]2[C@H]([C@@H]([C@@H]([C@@H](O2)C)O)O)O
Structure Classification
Initial Source

Human milk
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Lösungsmittel & Löslichkeit
In Vitro: 

H2O : 50 mg/mL (102.37 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0473 mL 10.2367 mL 20.4733 mL
5 mM 0.4095 mL 2.0473 mL 4.0947 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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