5x ELISA COATING BUFFER
Katalog-Nummer MBS238276-1L
Size : 1L
Marke : MyBiosource
5x ELISA COATING BUFFER Accessory Reagent
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS238276MBS238276 is an accessory reagent targeting 5x ELISA COATING BUFFER. More details
Product Overview
Online Datasheet in PDFProduct Name
5x ELISA COATING BUFFER, Accessory Reagent
Full Product Name
5x ELISA COATING BUFFER
Form/Format
5 X Concentrate
5 x concentrate - liquid
5 x concentrate - liquid
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Shelf Life: 24 months from date of despatch.
Shelf Life: 24 months from date of despatch.
Perservative Stabilisers
0.5% Sodium Azide
Intended Use||This item is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.
This item contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4 degree C for long term storage.
Instructions For Use||1. Dilute the required volume of this item 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.
2. Add antibody or antigen to the working solution and mix for 5-15 minutes.
3. Coat plate (suggested volume 100-200ul per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.
Intended Use||This item is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.
This item contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4 degree C for long term storage.
Instructions For Use||1. Dilute the required volume of this item 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.
2. Add antibody or antigen to the working solution and mix for 5-15 minutes.
3. Coat plate (suggested volume 100-200ul per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.
Buffer Solution
Phosphate buffered saline
Applications Tested/Suitable for 5x ELISA COATING BUFFER accessory reagent
ELISA (EIA)
Other Notes
Small volumes of 5x ELISA COATING BUFFER accessory reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.