Adgre1 Rat Monoclonal Antibody [Clone ID: CI:A3-1]

Specifications

Product Data
Clone Name	CI:A3-1
Applications	FC
Recommended Dilution	Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
Reactivities	Mouse
Host	Rat
Isotype	IgG2b
Clonality	Monoclonal
Immunogen	Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS-1 mouse myeloma cell line.
Specificity	This antibody recognises the Mouse F4/80 antigen, a 160 kD glycoprotein expressed by murine macrophages. The antibody binds to Macrophages from different sites including the peritoneal cavity, lung, spleen and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture. The antigen is not expressed by lymphocytes or polymorphonuclear cells.
Formulation	PBS, pH 7.4 Label: APC State: Lyophilized purified IgG fraction Stabilizer: 1% BSA, 5% Sucrose Preservative: 0.09% Sodium Azide Label: Allophycocyanin
Reconstitution Method	Restore with 1 ml distilled water.
Purification	Affinity Chromatography on Protein A
Conjugation	APC
Storage	Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Gene Name	adhesion G protein-coupled receptor E1
Database Link	Entrez Gene 13733 Mouse UniProt ID Q61549
Background	F4/80 antigen is a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with Human EGF module-containing Mucin-like Hormone Receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
Synonyms	Emr1, Gpf480
Reference Data