AGL Human shRNA Plasmid Kit (Locus ID 178)

Specifications

Product Data
Locus ID	178
Synonyms	GDE
Vector	pRS
E. coli Selection	Ampicillin
Mammalian Cell Selection	Puromycin
Format	Retroviral plasmids
Kit Components	AGL - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 178). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_000028, NM_000642, NM_000643, NM_000644, NM_000645, NM_000646, NM_000642.1, NM_000642.2, NM_000645.1, NM_000645.2, NM_000643.1, NM_000643.2, NM_000644.1, NM_000644.2, NM_000028.1, NM_000028.2, NM_000646.1, NM_000646.2, BC078663, BC078663.1, BC020705, BC057220, NM_000642.3
UniProt ID	P35573
Summary	This gene encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact tech@clinisciences.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at tech@clinisciences.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).