Specifications

Product Data
Locus ID	11651
Synonyms	Ak; Akt; LTR-akt; PK; PKB; PKB/A; PKB/Akt; PKBalpha; Rac
Vector	pGFP-C-shLenti
E. coli Selection	Chloramphenicol (34 ug/ml)
Mammalian Cell Selection	Puromycin
Format	Lentiviral plasmids
Components	Akt1 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 11651). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	BC066018, NM_001165894, NM_009652, NM_009652.1, NM_009652.2, NM_009652.3, NM_001165894.1, BC115583
UniProt ID	P31750
Summary	This gene encodes the founding member of the Akt serine-threonine protein kinase gene family that also includes Akt2 and Akt3. This kinase is a major downstream effector of the phosphatidylinositol 3-kinase (PI3K) pathway that mediates the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). It is activated through recruitment to cellular membranes by PI3K lipid products and by phosphorylation by 3-phosphoinositide dependent kinase-1. It then further phosphorylates different downstream proteins in response to various extracellular signals and thus plays a pivotal role in mediating a variety of cellular processes, such as glucose metabolism, glycogen biosynthesis, protein synthesis and turn over, inflammatory response, cell survival (anti-apoptosis) and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2009
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact tech@clinisciences.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Storage	Store at -20°C
Shipping	Ambient

Product Manuals

HuSH-29 shRNA Plasmids HuSH pRS Cloning Vector HuSH RNAi Validation Vector HuSH pGFP-V-RS Cloning Vector

FAQs

shRNA (HuSH-29)

SDS

SDS for TL512231

RNAi Resources

Product overview Video shRNA Selection Guide FAQ: shRNA FAQ: siRNA FAQ; miRNA Product Brochure: RNAi QC Data for Lenti shRNA