ASM10523-A565-100 | AMBRA1 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AMBRA1 Polyclonal Antibody (ASM10523). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-AMBRA1 Polyclonal Antibody (ASM10523) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) AMBRA1 Antibody (D) Composite.
Western blot analysis of Rat Brain cell lysates showing detection of 142.5 kDa AMBRA1 protein using Rabbit Anti-AMBRA1 Polyclonal Antibody (ASM10523). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AMBRA1 Polyclonal Antibody (ASM10523) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 142.5 kDa. Other Band(s): 50 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, ICC
Primary Accession	Q9C0C7
Other Accession	NP_001254711.1
Host	Rabbit
Reactivity	Human, Rat
Clonality	Polyclonal
Description	Rabbit Anti-Human AMBRA1 Polyclonal
Target/Specificity	Detects ~142 kDa.
Other Names	AMBRA1 Antibody, Activating molecule in BECN1-regulated autophagy protein 1 Antibody, KIAA1736 Antibody
Immunogen	Synthetic peptide from the C-terminal of Human AMBRA1 (aa. 202-212)
Purification	Peptide Affinity Purified
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:1000 dilution of SPC-644 was sufficient for detection of AMBRA1 in 15 µg of Rat Brain Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization	Cytoplasmic Vesicle \| Autophagosome

AMBRA1 Antibody