Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Anti-FOX1 Antibody

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Katalog-Nummer 581-FOX1

Size : 100uL

Marke : Phosphosolutions

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Anti-FOX1 Antibody (1G10)

Our Anti-FOX1 primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, and rat FOX1 and is Protein G purified. It is great for use in WB, IHC, ICC.

Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX1 protein.

Immunofluorescence of cultured rat neurons showing nuclear and distal cytoplasmic labeling of FOX1 (Cat. 581-FOX1, 1:500, green).

Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX1 protein.

Click on image to zoom

Species Reactivity

Bovine, Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 581-FOX1

Ships: 1-2 business days

Product Details

Target

FOX1

Scientific Background

FOX1, also known as ataxin-2 binding protein 1 (A2BP1), is a neuron specific RNA-binding protein that has been shown to regulate a wide range of alternative splicing events implicated in neuronal development and maturation including transcription factors and synaptic proteins (Fogel et al., 2012). Defects in FOX1 have also been associated with autism (Martin et al., 2007), attention-deficit hyperactivity disorder (Elia et al., 2010) and schizophrenia (Xu et al., 2008).

Format

Protein G Purified

Clonality

Monoclonal

Clone

1G10

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

RBFOX1

Molecular Weight

46/48 kDa

Immunogen

Recombinant protein taken from the N-terminus of human FOX1 expressed in and purified from E. Coli.

Immunogen Species

Human

Species Reactivity

Bovine, Human, Mouse, Rat

Antibody Registry ID

AB_2492095

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant.

Buffer

PBS + 10 mM NaN3.

Dilution Ranges

WB: 1:5000
WB Brain: 1:5000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~46/48 kD FOX1 protein doublet.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

2BP1 antibody, A2 BP1 antibody, A2BP 1 antibody, A2BP antibody, A2BP1 antibody, Ataxin 2 binding protein 1 antibody, Ataxin-2-binding protein 1 antibody, Ataxin2 binding protein 1 antibody, FOX1 antibody, Fox-1 homolog A antibody, fox-1-like RNA-binding protein 1 antibody, Hexaribonucleotide binding protein 1 antibody, Hexaribonucleotide-binding protein 1 antibody, HRNBP 1 antibody, HRNBP1 antibody, RBFOX1 antibody, RFOX1_HUMAN antibody, RNA binding protein fox-1 homolog 1 antibody, RNA binding protein fox-1 homolog 1 C. elegans, homolog of 1 antibody, RNA binding protein fox-1 homolog (C. elegans) 1 antibody

UniProt ID

Q9NWB1

NCBI Gene ID

54715

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Elia, J., Gai, X., Xie, H.M., Perin, J.C., Geiger, E., Glessner, J.T., D'arcy, M., Deberardinis, R., Frackelton, E., Kim, C. and Lantieri, F., 2010. Rare structural variants found in attention-deficit hyperactivity disorder are preferentially associated with neurodevelopmental genes. Molecular Psychiatry, 15(6), p.637-46. PMID: 19546859
Fogel, B.L., Wexler, E., Wahnich, A., Friedrich, T., Vijayendran, C., Gao, F., Parikshak, N., Konopka, G. and Geschwind, D.H., 2012. RBFOX1 regulates both splicing and transcriptional networks in human neuronal development. Human Molecular Genetics, 21(19), pp.4171-4186. PMID: 22730494
Martin, C.L., Duvall, J.A., Ilkin, Y., Simon, J.S., Arreaza, M.G., Wilkes, K., Alvarez‐Retuerto, A., Whichello, A., Powell, C.M., Rao, K. and Cook, E., 2007. Cytogenetic and molecular characterization of A2BP1/FOX1 as a candidate gene for autism. American Journal of Medical Genetics Part B: Neuropsychiatric Genetics, 144(7), pp.869-876. PMID: 17503474
Xu, B., Roos, J.L., Levy, S., Van Rensburg, E.J., Gogos, J.A. and Karayiorgou, M., 2008. Strong association of de novo copy number mutations with sporadic schizophrenia. Nature Genetics, 40(7), p.880-5. PMID: 18511947