General Information
| Short Description | Rabbit monoclonal GATA3 (1-200) antibody for use in WB, IP, ELISA, ChIP and ChIP-seq in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA/ChIP/ChIP-seq |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S0MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:6000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.ChIP:5 Mu g ant |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Immunogen Region | 1-200 |
| Immunogen Sequence | MEVTADQPRWVSHHHPAVLN GQHPDTHHPGLSHSYMDAAQ YPLPEEVDVLFNIDGQGNHV PPYYGNSVRATVQRYPPTHH GSQVCRPPLLHGSLPWLDGG KALGSHHTASPWNLSPFSKT SIHHGSPGPLSVYPPASSSS LSGGHASPHLFTFPPTPPKD VSPDPSLSTPGSAGSARQDE KECLKYQVPLPDSMKLESSH |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human GATA3 (P23771). |
Additional Info
| Tissue Specificity | T-cells and endothelial cells. |
| Function | Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression. Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages. |
| Protein Name | Trans-Acting T-Cell-Specific Transcription Factor Gata-3Gata-Binding Factor 3 |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Trans-Acting T-Cell-Specific Transcription Factor Gata-3 antibodyAnti-Gata-Binding Factor 3 antibodyAnti-GATA3 antibody |
Information sourced from Uniprot.org