Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Anti-Glyceraldehyde 3-Phosphate Dehydrogenase Antibody

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Katalog-Nummer 600-GAPDH

Size : 100uL

Marke : Phosphosolutions

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Anti-Glyceraldehyde 3-Phosphate Dehydrogenase Antibody (1D4)

Our Anti-Glyceraldehyde 3-Phosphate Dehydrogenase primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Glyceraldehyde 3-Phosphate Dehydrogenase and is Protein G purified. It is great for use in WB, IHC, ICC.

Immunostaining of HeLa cells labeled with Anti-GAPDH (Cat. 600-GAPDH, 1:100, green) and nuclear staining with DAPI (blue). The anti-GAPDH produces strong cytoplasmic labeling of healthy cells.

Western blot of rat hippocampal lysate showing the specific immunolabeling of ~38 kDa GAPDH protein.

Immunostaining of Human neuroblastoma SH-SY5Y cells labeled with Anti-GAPDH (Cat. 600-GAPDH, 1:100, green), Anti NF-H (Cat. 1451-NFH , red, 1:25,000) and nuclear staining with DAPI (blue).

Immunostaining of HeLa cells labeled with Anti-GAPDH (Cat. 600-GAPDH, 1:100, green) and nuclear staining with DAPI (blue). The anti-GAPDH produces strong cytoplasmic labeling of healthy cells.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 600-GAPDH

Ships: 1-2 business days

Product Details

Target

Glyceraldehyde 3-Phosphate Dehydrogenase

Scientific Background

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription (1). GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease (2,3). Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic (4).

Format

Protein G Purified

Clonality

Monoclonal

Clone

1D4

Isotype

IgM

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

GAPDHS

Molecular Weight

38 kDa

Immunogen

Purified porcine GAPDH.

Immunogen Species

Porcine

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2492132

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant

Buffer

PBS + 50% glycerol and 5 mm NaN3

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:100
ICC: 1:100

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~38 kDa Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

38 kDa BFA-dependent ADP-ribosylation substrate antibody, aging associated gene 9 protein antibody, Aging-associated gene 9 protein antibody, BARS-38 antibody, cb609 antibody, EC 1.2.1.12 antibody, Epididymis secretory sperm binding protein Li 162eP antibody, G3P_HUMAN antibody, G3PD antibody, G3PDH antibody, GAPD antibody, GAPDH antibody, Glyceraldehyde 3 phosphate dehydrogenase antibody, Glyceraldehyde-3-phosphate dehydrogenase antibody, HEL-S-162eP antibody, KNC-NDS6 antibody, MGC102544 antibody, MGC102546 antibody, MGC103190 antibody, MGC103191 antibody, MGC105239 antibody, MGC127711 antibody, MGC88685 antibody, OCAS, p38 component antibody, OCT1 coactivator in S phase, 38-KD component antibody, peptidyl cysteine S nitrosylase GAPDH antibody, Peptidyl-cysteine S-nitrosylase GAPDH antibody, wu:fb33a10 antibody

UniProt ID

P00355

NCBI Gene ID

26330

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Morgenegg, G., Winkler, G.C., Hübscher, U., Heizmann, C.W., Mous, J. and Kuenzle, C.C., 1986. Glyceraldehyde‐3‐phosphate dehydrogenase is a nonhistone protein and a possible activator of transcription in neurons. Journal of Neurochemistry, 47(1), pp.54-62. PMID: 7859286
Burke, J.R., Enghild, J.J., Martin, M.E., Jou, Y.S., Myers, R.M., Roses, A.D., Vance, J.M. and Strittmatter, W.J., 1996. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH. Nature Medicine, 2(3), p.347. PMID: 7859286
Schulze, H., Schuler, A., Stüber, D., Döbeli, H., Langen, H. and Huber, G., 1993. Rat brain glyceraldehyde‐3‐phosphate dehydrogenase interacts with the recombinant cytoplasmic domain of Alzheimer's β‐amyloid precursor protein. Journal of Neurochemistry, 60(5), pp.1915-1922. PMID: 7859286
Dastoor, Z. and Dreyer, J.L., 2001. Potential role of nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase in apoptosis and oxidative stress. Journal of Cell Science, 114(9), pp.1643-1653. PMID: 7859286