Anti-NANOG Antibody (5J818)
Katalog-Nummer NB-64-118092-100uL
Size : 100uL
Marke : Neo Biotech
Remove All
Anti-NANOG Antibody (5J818)
Copy Product InfoSynonyms:
Catalog No. TMAC-02746 Copy Product Info
Anti-NANOG Antibody (5J818) is a Rabbit antibody targeting NANOG. Anti-NANOG Antibody (5J818) can be used in FCM,ICC/IF,IHC,WB.
For research use only—not for human use. No sales to individuals. Use as intended only.
Product Information
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-NANOG Antibody (5J818) is a Rabbit antibody targeting NANOG. Anti-NANOG Antibody (5J818) can be used in FCM,ICC/IF,IHC,WB. |
| Ig Type | IgG |
| Clone | 5J818 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Western blot analysis of Nanog on F9 cell lysates using anti-Nanog antibody at 1/1,000 dilution. 2. ICC staining Nanog in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. Flow cytometric analysis of NCCIT cells with Nanog antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.  , , |
| Application | FCM ICC/IF IHC WB |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Nanog (from ??Tir Na Nog,?? the mythologic Celtic land of the ever young) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression have been detected by Northern analysis in the undifferentiated NTERA-2 cl.D1 embryonal carcinoma cell line. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID | Q9H9S0 |
| Molecular Weight | Theoretical: 37 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Calculator
Keywords
NANOGHomeobox transcription factor Nanog (hNanog)Homeobox protein NANOGhNanog
Preview
Related Tags: Anti-NANOG Antibody (5J818) molecular weight
Generate Quote
Catalog No.: Cas No.:
Add
| Size | Quantity | Unit Price | Amount | Operation |
|---|
Generate Quote