Mouse anti-Human/Mouse/Rat/Guinea Pig/Gerbil/Cat/Pig/Rabbit/Cow/Chicken Nucleolin Antibody
Anti-Nucleolin Antibody
Anti-Nucleolin Antibody
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Product Description
Specifications
| Size | 20 µg, 100 µg |
| Species | Human, Mouse, Rat |
| Specificity | Other species not tested. |
| Host Species | Mouse |
| Accession Number | P19338 |
| Gene Id | 4691 |
| Gene Symbols | NCL |
| Protein Name / Synonyms | Nucleolin (Protein C23) |
| Immunogen | Recombinant human protein was used as the immunogen for the Nucleolin antibody. |
| Isotype | IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
| Recommended Applications | Flow Cytometry: 0.5-1ug/million cells in 0.1ml IF: 0.5-1ug/ml Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT (1) Western blot: 0.5-1ug/ml Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Nucleoli |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.
Storage
Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.