Anti-PGC1 alpha Antibody
Katalog-Nummer MBS8307747-0,1mL
Size : 0.1mL
Marke : MyBiosource
Western blot analysis of PGC1 alpha expression in mouse heart (A), rat heart (B) whole cell lysates
Immunofluorescent analysis of PGC1 alpha staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunohistochemical analysis of PGC1 alpha staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
PGC1 Alpha (PGC1A) Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8307747MBS8307747 is an antibody targeting PGC1A. Also known as Anti-PGC1 alpha Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. Rabbit polyclonal antibody to PGC1 alpha More details
Product Overview
Online Datasheet in PDFProduct Name
PGC1 alpha (PGC1A), Polyclonal Antibody
Full Product Name
Anti-PGC1 alpha Antibody
Product Gene Name
anti-PGC1A antibody
Product Synonym Gene Name
PPARGC1A
Product Synonym Names
LEM6; PGC1; PPARGC1; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha; Ligand effect modulator 6
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Purity/Purification
Purified by Immunogen Affinity Chromatography
Specificity
Recognizes endogenous levels of PGC1 alpha protein.
Preparation and Storage
Upon receipt aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Molecular Weight
Predicted band size: 14; 30; 31; 33; 77; 89; 91 kD
Observed band size: 91 kD
Observed band size: 91 kD
Chromosome Location
Chromosome: 4; NC_000004.11 (23793644..24474394, complement). Location: 4p15.1
OMIM
604517
Immunogen
KLH-conjugated synthetic peptide of human PGC1 alpha. The exact sequence is proprietary.
Applications Tested/Suitable for anti-PGC1A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-PGC1A antibody
WB: 1:500-1:1000
IHC: 1:50-1:100
IF/ICC: 1:50-1:100
IHC: 1:50-1:100
IF/ICC: 1:50-1:100
Immunofluorescence (IF) of anti-PGC1A antibody
Immunofluorescent analysis of PGC1 alpha staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunohistochemistry (IHC) of anti-PGC1A antibody
Immunohistochemical analysis of PGC1 alpha staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Other Notes
Small volumes of anti-PGC1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for PGC1A. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
peroxisome proliferator-activated receptor gamma coactivator 1-alpha
NCBI Official Synonym Full Names
peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
NCBI Official Symbol
PPARGC1A
NCBI Official Synonym Symbols
LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1(alpha)
UniProt Protein Name
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
UniProt Synonym Protein Names
Ligand effect modulator 6
UniProt Gene Name
PPARGC1A
UniProt Synonym Gene Names
LEM6; PGC1; PGC1A; PPARGC1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha
UniProt Entry Name
PRGC1_HUMAN
NCBI Protein Information
peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC-1-alpha; L-PGC-1alpha; PPARGC-1-alpha; ligand effect modulator-6; PPAR gamma coactivator variant form; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcrip
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-PGC1A antibody
| Reference Product | PubMed Publications |
|---|---|
| PPARG antibody | >231 publications with PGC1A and PPARG |
| SIRT1 antibody | >107 publications with PGC1A and SIRT1 |
| HNF4A antibody | >26 publications with PGC1A and HNF4A |
| PPARA antibody | >16 publications with PGC1A and PPARA |
| NCOA1 antibody | >14 publications with PGC1A and NCOA1 |
| ESRRG antibody | >12 publications with PGC1A and ESRRG |
| CREBBP antibody | >8 publications with PGC1A and CREBBP |
| ESRRA antibody | >5 publications with PGC1A and ESRRA |
| EP300 antibody | >2 publications with PGC1A and EP300 |
Pathways associated with anti-PGC1A antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| ATF-2 Transcription Factor Network Pathway antibodies | ATF-2 Transcription Factor Network Pathway Diagram |
| Adipocytokine Signaling Pathway antibodies | Adipocytokine Signaling Pathway Diagram |
| Adipocytokine Signaling Pathway antibodies | Adipocytokine Signaling Pathway Diagram |
| Adipogenesis Pathway antibodies | Adipogenesis Pathway Diagram |
| BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway antibodies | BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway Diagram |
| Circadian Clock Pathway antibodies | Circadian Clock Pathway Diagram |
| Circadian Repression Of Expression By REV-ERBA Pathway antibodies | Circadian Repression Of Expression By REV-ERBA Pathway Diagram |
| Developmental Biology Pathway antibodies | Developmental Biology Pathway Diagram |
| Energy Metabolism Pathway antibodies | Energy Metabolism Pathway Diagram |
| Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies | Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram |
Diseases associated with anti-PGC1A antibody
| Disease Name | Pubmed Publications |
|---|---|
| Insulin Resistance Antibodies | >186 publications with PGC1A and Insulin Resistance |
| Cardiovascular Diseases Antibodies | >132 publications with PGC1A and Cardiovascular Diseases |
| Disease Models, Animal Antibodies | >113 publications with PGC1A and Disease Models, Animal |
| Fatty Liver Antibodies | >101 publications with PGC1A and Fatty Liver |
| Diabetes Mellitus, Type 2 Antibodies | >96 publications with PGC1A and Diabetes Mellitus, Type 2 |
| Heart Diseases Antibodies | >85 publications with PGC1A and Heart Diseases |
| Inflammation Antibodies | >80 publications with PGC1A and Inflammation |
| Liver Diseases Antibodies | >73 publications with PGC1A and Liver Diseases |
| Neoplasms Antibodies | >67 publications with PGC1A and Neoplasms |
| Hyperglycemia Antibodies | >57 publications with PGC1A and Hyperglycemia |
Organs/Tissues associated with anti-PGC1A antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Muscle Antibodies | >631 publications with PGC1A and Muscle |
| Liver Antibodies | >306 publications with PGC1A and Liver |
| Heart Antibodies | >176 publications with PGC1A and Heart |
| Connective Tissue Antibodies | >140 publications with PGC1A and Connective Tissue |
| Brain Antibodies | >115 publications with PGC1A and Brain |
| Kidney Antibodies | >36 publications with PGC1A and Kidney |
| Thyroid Antibodies | >28 publications with PGC1A and Thyroid |
| Pancreas Antibodies | >25 publications with PGC1A and Pancreas |
| Embryonic Tissue Antibodies | >15 publications with PGC1A and Embryonic Tissue |
| Intestine Antibodies | >15 publications with PGC1A and Intestine |