Anti-Polysialic acid [735]

Katalog-Nummer MBS488177-0,1mg

Size : 0.1mg

Marke : MyBiosource


Polysialic Acid Recombinant Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS488177

Recombinant monoclonal antibody to Polysialic acid. Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 735. More details

Product Overview

Online Datasheet in PDF
Product Name
Polysialic acid, Recombinant Antibody
Full Product Name
Anti-Polysialic acid [735]
Product Synonym Names
PSA; N-CAM; neural cell adhesion molecule; polySA
Isotype
IgG, kappa
Clone Number
735
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS with 0.02% Proclin 300.
Purity/Purification
Protein A Affinity Purified
Concentration
1 mg/mL (lot specific)
Specificity
Recognizes Polysialic acid (PSA), a carbohydrate polymer attached to the neural cell adhesion molecule (NCAM), which shares molecular epitopes with homopolymers of α2,8-linked sialic acid of Escherichia coli K1.
Preparation and Storage
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Animal-free Manufacturing Platform
The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free.
Immunogen
Homopolymers of α2, 8-linked sialic acid of Escherichia coli K1.

Product Categories/Family for Polysialic acid recombinant antibody

Neurobiology; Development; Cancer
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for Polysialic acid recombinant antibody
Immunoblot, Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC), Immunohistochemistry (IHC)
Application Notes for Polysialic acid recombinant antibody
Clone 735 was used for immunostaining and immunoprecipitation in an experimental setup involving antigen preparation from PBMC (Peripheral Blood Mononuclear Cells) (Husmann et al., 1989; PMID: 2529126). This antibody recognises an unusual postranslational modification on neural cell adhesion molecules important in synaptogenesis, oncogenesis (particularly small-cell lung cancer) and development of organs such as the kidney (studied in rats, Lackie et al., 1990). 735 was used for Western blot analysis of PSA-NCAM (Polysialylated Neural Cell Adhesion Molecule) (Charter et al., 2000; PMID: 11030751). In addition to various studies on human cells, the antibody, has also been used the study the fine-composition of poly-sialic acid composition in mice (Galuska et al., 2006).

Immunohistochemistry (IHC) of Polysialic acid recombinant antibody

Immunohistochemical staining of guinea pig brain using anti-polysialic acid antibody MBS488177 Formalin fixed guinea pig brain slices were were stained with MBS488177 at 3 µg/ml.
Rabbit Anti-Polysialic Acid Recombinant Antibody – Cat# MBS488177

Immunohistochemistry (IHC) of Polysialic acid recombinant antibody

Immunohistochemical staining of rat spinal chord using anti-polysialic acid antibody MBS488177 Formalin fixed rat spinal chord slices were were stained with a MBS488177 at 3 µg/ml.
Rabbit Anti-Polysialic Acid Recombinant Antibody, Immunohistochemistry (IHC) – Cat# MBS488177

Western Blot (WB) of Polysialic acid recombinant antibody

Western Blot using anti-polysialic acid antibody MBS004198 Rat brain lysate was resolved on a 10% SDS PAGE gel and blots probed with MBS48177 at 2 µg/ml before being detected by a secondary antibody.
Rabbit Anti-Polysialic Acid Recombinant Antibody, Western Blot (WB) – Cat# MBS488177

Product References and Citations for Polysialic acid recombinant antibody

Bitter-Suermann & Roth EMonoclonal antibodies to polysialic acid reveal epitope sharing between invasive pathogenic bacteria, differentiating cells and tumor cells. Immunol Res. 1987; 6(4):225-37. PMID:2448401

Other Notes

Small volumes of Polysialic acid recombinant antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Polysialic acid purchase

MBS488177 is a recombinant antibody targeting Polysialic acid. Also known as Anti-Polysialic acid [735]. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Polysialic acid. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
polysialic acid capsule biosynthesis sialic acid synthase NeuB
Protein Family
Polysialic acid biosynthesis protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

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