Anti-Rat MAG (Clone 118423)
Katalog-Nummer M1279-25
Size : 25ug
Marke : Leinco Technologies
AntiRat MAG [Clone 118423]
AntiRat MAG [Clone 118423]
Product No.: M1279
Clone 118423 Target MAG Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Siglec4a, GMA Isotype IgG2a Applications N , WB |
Antibody DetailsProduct DetailsReactive Species Rat Host Species Mouse Immunogen NS0Derived Recombinant Rat MAG Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at 20°C to 70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 28°C. For longterm storage of the reconstituted antibody, aseptically aliquot into working volumes and store at 20°C to 70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831273 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Rat MAG this monoclonal antibody can be used at a concentration of 12 µg/ml. This monoclonal antibody should be used in conjunction with compatible secondstep reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Rat MAG is 25 ng/lane under either reducing or nonreducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Rat MAG bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat MAG is 40 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse AntiRat MyelinAssociated Glycoprotein (MAG) (Clone 118423) recognizes an epitope on Rat MAG. This monoclonal antibody was purified using multistep affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Myelinassociated glycoprotein (MAG) is a transmembrane proteins that is a component of myelin in the central and peripheral nervous system.1 It promotes neurite outgrowth during the embryonic development, but inhibits axonal regeneration in the adult nervous system.2 MAG mediates cellcell interactions between myelinating glial cells and neurons.3 MAG as an important component of the myelinderived inhibition of nerve regeneration. The acute loss of MAG function can promote significant axon growth across a site of CNS nerve damage.4 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Ming, GL. et al. (2008) Mol Brain. 1:10 2. Kelm, S. et al. (2001) FEBS Lett. 499: 262 3. Del Rio, JA. et al. (2005) Mol Cell Neurosci. 29: 471 4. Jay, DG. et al. (2003) J of Neurosci. 23: 3112 |