Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Anti-Talin (Ser-425), Phosphospecific Antibody

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Katalog-Nummer TP4171

Size : 100uL

Marke : Phosphosolutions

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Anti-Talin (Ser-425), Phosphospecific Antibody

Our Talin (Ser-425) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Talin (Ser-425) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot of rat PC12 cells stimulated with Calyculin A (100 nM) for 30 min (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin (8D4) (lanes 1 & 2) or rabbit polyclonal anti-Talin (Ser-425) (lanes 3 & 4) antibodies.

Immunocytochemical labeling of Talin phosphorylation relative to F-actin in chick fibroblasts. The cells were labeled with rabbit polyclonal Talin (Ser-425) antibody (TP4171), then the antibody was detected using appropriate secondary antibody (Green). This labeling is compared to F-actin staining (Red, Left). (Image provided by Dr. Gianluca Gallo at Drexel University).

Representative WB of whole cell lysates from sh-SCR and sh-ARRB1 HOFs stimulated or not with ET-1 (5 min) and probed with antibodies to active or total Intβ1, b-arr1, pTalin (Cat. TP4171) and Talin. Image from publication CC-BY-4.0. PMID: 38777849

Western blot of rat PC12 cells stimulated with Calyculin A (100 nM) for 30 min (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin (8D4) (lanes 1 & 2) or rabbit polyclonal anti-Talin (Ser-425) (lanes 3 & 4) antibodies.

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Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: TP4171

Ships: 1-2 business days

Product Details

Target

Talin (Ser-425)

Scientific Background

Talin is an important cytoskeletal component of integrin adhesion sites. Calpins cleave talin precursor (240 kDa) into an amino-terminal globular head domain of 47 kDa and a carboxyl-terminal 190 kDa rod domain. The talin head domain contains a FERM domain that binds integrins, PIP kinase (Type I), and FAK. The rod domain has several vinculin-binding sites, a second integrin-binding site, and two actin-binding sites. These talin protein-protein interactions are critical for integrin activation, focal adhesion formation, and cell migration. Talin regulation may occur through phosphorylation and regulated degradation. The talin head domain binds Smurf1, an E3 ubiquitin ligase, and this interaction leads to talin head ubiquitylation and degradation. Cdk5 can phosphorylate Ser-425 in the head domain, and this inhibits both binding to Smurf1 and subsequent degradation. The S425A talin mutant resists Cdk5 phosphorylation, increases susceptibility to Smurf1-mediated ubiquitylation, and inhibits cell migration. Thus, talin head phosphorylation may be important for regulating adhesion stability and cell migration

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

TLN1

Molecular Weight

240

Immunogen

Phospho-Talin (Ser-425) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-425 in human Talin 1. This sequence is conserved in rat, mouse, and chicken Talin 1, as well as in Talin 2 (Ser-428).

Immunogen Species

Human

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:200

Conjugate

Unconjugated

Specificity

The antibody detects a 240 kDa* protein corresponding to the molecular mass of Talin on SDS-PAGE immunoblots of human A431, rat PC12, and rabbit fibroblast cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-425

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

TLN1

UniProt ID

Q9Y490

Shipping

Blue Ice

Anti-Talin (Ser-425), Phosphospecific Antibody

Katalog-Nummer TP4171

Marke : Phosphosolutions

Anti-Talin (Ser-425), Phosphospecific Antibody

SKU: TP4171

Product Details

Bulk Order Anti-Talin (Ser-425), Phosphospecific Antibody