Anti-TLR2 Antibody (7Y355)

Anti-TLR2 Antibody (7Y355) is a Rabbit antibody targeting TLR2. Anti-TLR2 Antibody (7Y355) can be used in FCM, WB.

IgG

7Y355

Human,Mouse

1. THP-1 (H) cells were treated with or without LPS (1 μg/ml) for 24 h, 25 μg total protein per Lane of cell lysates probed with TLR2 monoclonal antibody, unconjugated (TMAB-01853) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. The THP-1 (H) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-TLR2 antibody (TMAB-01853): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Blank control (black): PBS.
3. The Raw264.7 (M) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-TLR2 antibody (TMAB-01853): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Blank control (black): PBS.

FCM=1 μg/Test; WB=1:1000-5000

Monoclonal

Recombinant Protein: full length protein of human TLR2

Human

TLR2

Toll-like receptor 2

Inflammatory mediators,Receptors,Macrophage / Inflamm.,TLR Signaling,Defensin,Transmembrane

Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.