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> Apolipoprotein E/APOE3 Protein, Human, Recombinant (hFc)

Apolipoprotein E/APOE3 Protein, Human, Recombinant (hFc)

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Katalog-Nummer NB-64-54495-200ug

Size : 200ug

Marke : Neo Biotech


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Apolipoprotein E/APOE3 Protein, Human, Recombinant (hFc)

(Synonyms: Apolipoprotein E, apolipo E, APOE3, Apo-E, APOE) Copy Product Info

Synonyms: Apolipoprotein E, apolipo E, APOE3, Apo-E, APOE

Catalog No. TMPK-00762 Copy Product Info
Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD). Apolipoprotein E/APOE3 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with N-hFc tag. The predicted molecular weight is 59.76 kDa and the accession number is P02649.
Other Forms of Apolipoprotein E/APOE3 Protein, Human, Recombinant (hFc):
Apolipoprotein E/APOE3 Protein, Human, Recombinant (His)
Apolipoprotein E/APOE Protein, Mouse, Recombinant (His)
Apolipoprotein E/APOE4 Protein, Human, Recombinant (hFc)
Apolipoprotein E/APOE4 Protein, Human, Recombinant (His)
Apolipoprotein E/APOE3 Protein (Primary Amine Labeling), Human, Recombinant (His), Biotinylated
Apolipoprotein E/APOE Protein, Mouse, Recombinant (hFc)
Apolipoprotein E/APOE3 Protein, Human, Recombinant (His & Trx)
Apolipoprotein E/APOE3 Protein, Human, Recombinant (His & Trx), Biotinylated
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
Human TREM2, His Tag captured on CM5 Chip via anti-his antibody can bind Human APOE3, hFc Tag with an affinity constant of 0.41 μM as determined in SPR assay.
Description
Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD). Apolipoprotein E/APOE3 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with N-hFc tag. The predicted molecular weight is 59.76 kDa and the accession number is P02649.
Species
Human
Expression System
HEK293 Cells
TagN-hFc
Accession NumberP02649
ConstructionLys19-His317
Protein Purity
> 95% as determined by Tris-Bis PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsApolipoprotein E, apolipo E, APOE3, Apo-E, APOE
Research Background
Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD).
Chemical Properties
Molecular Weight59.76 kDa (predicted). Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

APOE-3APOE 3
Related Tags: Apolipoprotein E/APOE3 Protein, Human, Recombinant (hFc) chemical structure | Apolipoprotein E/APOE3 Protein, Human, Recombinant (hFc) molecular weight