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> Aspartate Aminotransferase (AST/GOT) Activity Colorimetric Assay Kit

Aspartate Aminotransferase (AST/GOT) Activity Colorimetric Assay Kit

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Katalog-Nummer orb1173220

Size : 96T

Marke : Biorbyt


Aspartate Aminotransferase (AST/GOT) Activity Colorimetric Assay Kit

SKU: orb1173220

Description

This kit detects AST/GOT activity by catalyzing the transamination of α-ketoglutarate and L-aspartic acid to produce pyruvate. The rate of absorbance increase at 505 nm is used to calculate AST/GOT activity.

Images & Validation

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Aspartate Aminotransferase (AST/GOT) Activity Colorimetric Assay Kit
Application Notes
The kit provides a simple method for detecting AST/GOT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, Aspartate aminotransferase (AST) catalyzes the transamination reaction of α-ketoglutarate and L-aspartic acid at 37°C and pH 7.4 to produce glutamic acid and pyruvate; Pyruvate can interact with 2,4- Dinitrophenylhydrazine to produce pyruvate phenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of pyruvate phenylhydrazone increase at 505 nm can reflect AST/GOT activity.

Key Properties

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Storage & Handling

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StorageStorage at 4°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.
Expiration Date6 months from date of receipt.
Hazard InformationThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
DisclaimerFor research use only

Alternative Names

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AST; GOT; Aspartate aminotransferase

Protocol Information