Bauhinia purpurea Lectin (BPL)

This tetrameric lectin consists of 44,000D subunits and does not require divalent cations for binding. Binding appears to be highest for glycoconjugates containing galactosyl (b-1,3) N-acetylgalactosamine structures but oligosaccharides with a terminal alpha linked N-acetylgalactosamine can also bind. Although binding specificity is similar to that of peanut agglutinin, tissue staining patterns of these two lectins are distinct.

Sugar Specificity:
Terminal β-Gal and terminal β-GalNAc

Inhibiting/Eluting Sugar:
100mM lactose

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.