Calmodulin-tag Binding/ Wash Buffer
Catalog Number: B2016319 (50 mL)
Calmodulin-tag Binding/ Wash Buffer is a high quality buffer solution used in protein purification techniques, particularly in affinity chromatography to bind and wash proteins that have been tagged with calmodulin, a calcium-binding protein. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.
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Product Description
Calmodulin-tag Binding/ Wash Buffer
Catalog number: B2016319
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 mL
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: RT
Keywords: Calmodulin Binding/ Wash Buffer
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.
References:
1: Li CQ, Ye P, Cao Zf, Wang H, Lu L, Nicastro P, Wood E, Robert JJ, Ouwehand WH, Hill F, López JA, Wardell MR. Expression of the amino-terminal domain of platelet glycoprotein Ib alpha: exploitation of a calmodulin tag for determination of its functional activity Protein Expr Purif. 2001 Jul;22(2):200-10.
2: Chad JE, Eckert R. An enzymatic mechanism for calcium current inactivation in dialysed Helix neurones J Physiol. 1986 Sep;378:31-51.
3: Terpe K. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems Appl Microbiol Biotechnol. 2003 Jan;60(5):523-33.
4: Murray G, Marshall MJ, Trumble W, Magnuson BA. Calmodulin-binding protein detection using a non-radiolabeled calmodulin fusion protein Biotechniques. 2001 May;30(5):1036-42.
5: Lin L, Liu C, Nayak BC, He W, You M, Yuchi Z. A two-step purification strategy using calmodulin as an affinity tag J Chromatogr A. 2018 Apr 6;1544:16-22.
6: Mukherjee S, Ura M, Hoey RJ, Kossiakoff AA. A New Versatile Immobilization Tag Based on the Ultra High Affinity and Reversibility of the Calmodulin-Calmodulin Binding Peptide Interaction J Mol Biol. 2015 Aug 14;427(16):2707-25.
7: Zhao X, Li G, Liang S. Several affinity tags commonly used in chromatographic purification J Anal Methods Chem. 2013;2013:581093.
8: Klein W. Calmodulin-binding peptide as a removable affinity tag for protein purification Methods Mol Biol. 2003;205:79-97.
9: Li Y. Commonly used tag combinations for tandem affinity purification Biotechnol Appl Biochem. 2010 Feb 15;55(2):73-83.
10: Battesti A, Bouveret E. Improvement of bacterial two-hybrid vectors for detection of fusion proteins and transfer to pBAD-tandem affinity purification, calmodulin binding peptide, or 6-histidine tag vectors Proteomics. 2008 Nov;8(22):4768-71.
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Additional Information
| Weight | 2.6 oz |
|---|---|
| Dimensions | 3.3 × 1.2 × 1.2 in |