Canine Brachyury protein (T) ELISA Kit
Katalog-Nummer AE16193DO-480
Size : 5x96tests
Marke : Abebio
Reagent Preparation
Results demonstration
Assay Procedure Summary
Certificate
Product Details
| Species Reactivity | Canine (Canis familiaris; Dog) |
| UniProt | Q9GL27 |
| Abbreviation | T |
| Alternative Names | RP11-459F1.1; MGC104817; TFT; OTTHUMP00000017588|T brachyury homolog|T brachyury-like|transcription factor T |
| Range | Request Information |
| Sensitivity | Request Information |
| Sample Type | Serum, Plasma, Other biological fluids |
| Detection Method | Sandwich |
| Analysis Method | Quantitive |
| Assay Duration | 1-4.5h |
| Sample Volume | 1 - 2 0 0 μ L |
| Detection Wavelengt | 450 nm |
Test principle
This assay employs a two-site sandwich ELISA to quantitate T in samples. An antibody specific for T has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any T present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for T is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of T bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview
Components
Reagents | Quantity | Reagents | Quantity |
Assay plate (96 Wells) | .label-data{font-weight:bold;min-width:100px;}.label-data,.data{display:inline-block;min-width:100px;}.description table{word-break:break-all;}.description table td{min-width:200px;}
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