Flow Cytometry
Surface staining of human peripheral blood cells with CD22 monoclonal antibody, clone MEM-01 (PE) (Cat # MAB5034). Cells in the lymphocyte gate were used for analysis.
- Specifications
Product Description
Mouse monoclonal antibody raised against native CD22.
Immunogen
Native purified CD22 from Raji Burkitt's lymphoma cell line.
Host
Mouse
Theoretical MW (kDa)
130
Reactivity
Human, Primates
Specificity
This antibody reacts with CD22 (BL-CAM), a 130 KDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes.
Form
Liquid
Conjugation
PE
Isotype
IgG1
Recommend Usage
Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Flow Cytometry
Surface staining of human peripheral blood cells with CD22 monoclonal antibody, clone MEM-01 (PE) (Cat # MAB5034). Cells in the lymphocyte gate were used for analysis. - Gene Info — CD22
- Interactomes
- Pathways
- Diseases
- Publication Reference
- CD22: an inhibitory enigma.
Walker JA, Smith KG.
Immunology 2007 Dec; 123(3):314.
- Distinct endocytic mechanisms of CD22 (Siglec-2) and Siglec-F reflect roles in cell signaling and innate immunity.
Tateno H, Li H, Schur MJ, Bovin N, Crocker PR, Wakarchuk WW, Paulson JC.
Molecular and Cellular Biology 2007 Jun; 27(16):5699.
- CD22: a multifunctional receptor that regulates B lymphocyte survival and signal transduction.
Tedder TF, Poe JC, Haas KM.
Advances in Immunology 2005 Oct; 88:1.
- CD22: an inhibitory enigma.