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> CD81 Recombinant Monoclonal Mouse Antibody (2304.81) – Biotium Choice

CD81 Recombinant Monoclonal Mouse Antibody (2304.81) – Biotium Choice

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Katalog-Nummer P014-1MG

Size : 200UL

Marke : Biotium

CD3e Mouse Monoclonal Antibody (SK7), CF®405M Conjugate - Biotium Choice

CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice

CD81 (clone 2304.81) is a validated recombinant mouse monoclonal antibody that recognizes the human tetraspanin protein CD81. The antibody belongs to the Biotium Choice list of select antibodies that have been formulated and validated in-house for optimal performance.

Conjugation
Size
Catalog #
Qty
25 tests
100 tests
1 mg
5 mg
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CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg>
CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 2Methanol-fixed MCF7 cells were stained with 1 ug/mL recombinant monoclonal mouse anti-CD81 (2304.81), detected with CF®568 goat anti-mouse secondary antibody and imaged by confocal microscopy.
CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 3Recombinant CD81(2304.81) antibody was conjugated to CF®568 and used to stain MCF-7 cell-derived EVs (left panel). EV staining was compared to the antibody alone in buffer (right panel). Staining was detected in the B610 (PI) channel.
CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 4Western detection of human CD81 in MCF-7 cell and EV lysates using CD81 Recombinant Mouse Monoclonal Antibody (2304.81) followed by goat anti-mouse CF®680. M: 2 uL Peacock™ Plus Prestained Protein Marker. 10 ug of MCF-7 cell lysate and 1 ug of MCF-7 EV lysate were loaded. The blot was imaged on a LI-COR Odyssey® infrared imaging system in the 700 channel.
CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 5Recombinant CD81(2304.81) antibody was conjugated to CF®405M and used to stain the surface of Jurkat cells (blue). Isotype control antibody conjugated to CF®405M is shown in gray. Staining was detected in the V450 (Pacific Blue) channel.
CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 6Recombinant CD81(2304.81) antibody was conjugated to CF®488A and used to stain the surface of U2-OS cells (green). Isotype control antibody conjugated to CF®488A is shown in gray. Staining was detected in the B525 (FITC) channel.
CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 7Recombinant CD81(2304.81) antibody was conjugated to CF®568 and used to stain the surface of MCF-7 cells (orange). Unstained cells are shown in gray. Staining was detected in the Y585 (PE) channel.
CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 8Recombinant CD81(2304.81) antibody was conjugated to CF®583R and used to stain the surface of MCF-7 cells (red). Unstained cells are shown in gray. Staining was detected in the Y610 (mCherry) channel.
CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 9Recombinant CD81(2304.81) antibody was conjugated to CF®647Plus and used to stain the surface of MCF-7 cells (pink). Isotype control antibody conjugated to CF®647Plus is shown in gray. Staining was detected in the R660 (APC) channel.
CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 10Recombinant CD81(2304.81) antibody was conjugated to APC and used to stain the surface of MCF-7 cells (pink). Isotype control antibody conjugated to APC is shown in gray. Staining was detected in the R660 (APC) channel.
CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 11Recombinant CD81(2304.81) antibody was conjugated to CF®700 and used to stain the surface of MCF-7 cells (teal). Isotype control antibody conjugated to CF®700 is shown in gray. Staining was detected in the R712 (APC-700) channel.
CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 12Recombinant CD81(2304.81) antibody was conjugated to RPE-Astral™775 and used to stain the surface of MCF-7 cells (teal). Isotype control antibody conjugated to RPE-Astral™775 is shown in gray. Staining was detected in the Y763 channel.
  1. CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg>
  2. CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 2
  3. CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 3
  4. CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 4
  5. CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 5
  6. CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 6
  7. CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 7
  8. CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 8
  9. CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 9
  10. CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 10
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  12. CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice <svg class="svgicon svg-verified"><use xlink:href="#svg-verified"></use></svg> - Image 12
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Product Description

CD81 (clone 2304.81) is a recombinant mouse monoclonal antibody that recognizes the human tetraspanin protein CD81. This antibody belongs to the Biotium Choice list of select antibodies that have been formulated and validated in-house for optimal performance. This antibody has been validated in flow cytometry and immunofluorescence microscopy. The antibody is available unlabeled or conjugated to CF® Dyes and Astral™ tandem dyes.

CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspanin transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Tetraspanin proteins CD9, CD63, and CD81, are the most common proteins used as exosome markers. Tetraspanins are plasma membrane proteins with many proposed functions, including activation and sorting of other membrane proteins. They are also thought to play a role in the targeting of proteins to multivesicular bodies (MVBs) and exosomes. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of exosomes, but their expression levels vary depending on the cell type of origin.

As an isotype control, we recommend Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331) for this antibody.

Exosome Stains and Antibodies

Biotium also offers ExoBrite™ CD81 Flow Antibody and ExoBrite™ CD81 Western Antibody that are validated for optimal detection of isolated exosomes by flow cytometry or western blotting. Learn more about our products for exosome research.

Discover Sharper Signals and Unmatched Panel Flexibility with Biotium Choice Antibodies
Powered by CF® Dyes and Astral Leap™ Tandem Dyes

Biotium Choice antibodies are carefully curated and validated in-house to offer exceptional signal-to-noise. Labeled with our advanced CF® Dyes and Astral Leap™ tandem dyes, they are our top-recommended antibodies for flow cytometry and other applications.

Biotium Choice Antibody Features

  • Robust and validated clones against common targets
  • Developed and optimized for flow cytometry and other applications
  • Conjugated to bright, photostable CF® Dyes for superior signal and clarity
  •  Also available with Astral Leap™ tandem dyes for expanding multiplexing
  • New antibody clones and dye conjugates continuously in development

View our full catalog of Biotium Choice antibodies

Identifying Biotium validated antibodies

Biotium scientists validate and optimize select antibodies in our catalog, marked with a check mark next to the product name. This symbol indicates the antibody is highly recommended for the specified application(s).

Look for this symbol next to the product name on product pages and search results

ConjugationEx/EmConc.SizeCatalog No.Dye Features
CF®405M416/452 nm100 ug/mL25 tests (125 uL)P014-405M-125CF®405M Features
100 tests (500 uL)P014-405M-500
CF®488A490/516 nm100 ug/mL25 tests (125 uL)P014-488A-125CF®488A Features
100 tests (500 uL)P014-488A-500
CF® 568562/584 nm100 ug/mL25 tests (125 uL)P014-568-125CF®568 Features
100 tests (500 uL)P014-568-500
CF® 583R585/609 nm100 ug/mL25 tests (125 uL)P014-583R-125CF®583R Features
100 tests (500 uL)P014-583R-500
RPE-Astral™616496, 546, 566/617 nm100 ug/mL25 tests (125 uL)P014-R616-125RPE-Astral™616 Features
100 tests (500 uL)P014-R616-500
CF® 647Plus652/668 nm100 ug/mL25 tests (125 uL)P014-647P-125CF®647Plus Features
100 tests (500 uL)P014-647P-500
CF®700696/721 nm100 ug/mL25 tests (125 uL)P014-700-125CF®700 Features
100 tests (500 uL)P014-700-500
RPE-Astral™775496, 546, 565/774 nm100 ug/mL25 tests (125 uL)P014-R775-125RPE-Astral™775 Features
100 tests (500 uL)P014-R775-500
R-PE496, 546, 565/576 nm100 ug/mL25 tests (125 uL)P014-RPE-125
100 tests (500 uL)P014-RPE-500
APC651/660 nm100 ug/mL25 tests (125 uL)P014-APC-125
100 tests (500 uL)P014-APC-500
Purified (BSA-free)N/A5 mg/mL1 mg (200 uL)P014-1MG
5 mg (1 mL)P014-5MG
Tech Tips
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Troubleshooting Tips for Fluorescence Staining
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Protocol: Immunofluorescence Staining of Cells for Microscopy
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Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst
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Tech Tip: Fluorescence Bleed-Through and Cross-Talk

Product Attributes

Antibody number
#P014
SwissProt
P60033
Biotium Choice Antibody
Primary
Antibody type
Biotium Choice, Primary
Clonality
Monoclonal, Recombinant Monoclonal
Host species
Mouse
Clone
2304.81
Isotype
IgG1, kappa
Antibody reactivity (target)
CD81
Synonyms
CD81; Target of the antiproliferative antibody 1 (TAPA-1); Tetraspanin-28; Tspan-28
Species reactivity
Baboon, Cynomolgus monkey, Human, Mouse (low reactivity), Non-human primates
Human gene symbol
CD81
Entrez gene ID
975
Unigene
54457
Molecular weight
26 kDa
Antibody target cellular localization
Exosomes/EVs, Plasma membrane
Cell/tissue expression
Exosomes, Hepatocytes, Lymphocytes, Monocytes/macrophages
Verified antibody applications
Flow (surface) (verified), IF (verified)
Shelf life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Positive control
Ramos, Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63, MCF-7, and human lymphocytes. Tonsil or liver tissue.
Antibody application notes
Immunofluorescence: 1 ug/mL, Western Blot: 0.1 ug/mL, Flow cytometry: 0.5-1 ug/million cells
Antibody research areas
Exosomes/EVs
Antibody/conjugate formulation
Conjugates: PBS/0.1% BSA/0.05% azide, Purified, BSA-free: 5 mg/mL in PBS/0.05% azide
Volume per assay
5 uL/test
Storage Conditions
Store at 2 to 8 °C
Regulatory status
For research use only (RUO)
Product origin
Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.

Documents, Protocols, SDS and COA

Datasheet
Datasheet Download
COA
Request the Certificate of Analysis

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