CD9 (clone 2310.9) is a recombinant mouse monoclonal antibody that recognizes the human tetraspanin protein CD9. This antibody belongs to the Biotium Choice list of select antibodies that have been developed and validated in-house for optimal performance. This antibody has been validated in flow cytometry, immunofluorescence microscopy, and in western blot. The antibody is available unlabeled or conjugated to CF^® Dyes and Astral™ tandem dyes.

CD9 is a type IV transmembrane glycoprotein with four transmembrane domains. CD9 on pre-B cells may play a role in cell-cell adhesion. In addition, CD9 may play a role in signal transduction mediated by interaction with low molecular weight GTP binding proteins. CD9 is expressed on early B cells, eosinophils, basophils and activated T cells and is a major component of the platelet cell surface. It is also expressed on most non-T acute lymphoblastic leukemia cells and on some acute myeloid and chronic lymphoid leukemia.

Tetraspanin proteins CD9, CD63, and CD81, are the most common proteins used as exosome markers. Tetraspanins are plasma membrane proteins with many proposed functions, including activation and sorting of other membrane proteins. They are also thought to play a role in the targeting of proteins to multivesicular bodies (MVBs) and exosomes. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of exosomes, but their expression levels vary depending on the cell type of origin.

As an isotype control, we recommend Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331) for this antibody.

Exosome Stains and Antibodies

Biotium also offers ExoBrite™ CD9 Flow Antibody and ExoBrite™ CD9 Western Antibody that are validated for optimal detection of isolated exosomes by flow cytometry or western blotting. Learn more about our products for exosome research.

Discover Sharper Signals and Unmatched Panel Flexibility with Biotium Choice Antibodies
Powered by CF^® Dyes and Astral Leap™ Tandem Dyes

Biotium Choice antibodies are carefully curated and validated in-house to offer exceptional signal-to-noise. Labeled with our advanced CF^® Dyes and Astral Leap™ tandem dyes, they are our top-recommended antibodies for flow cytometry and other applications.

Biotium Choice Antibody Features

• Robust and validated clones against common targets
• Developed and optimized for flow cytometry and other applications
• Conjugated to bright, photostable CF^® Dyes for superior signal and clarity
•  Also available with Astral Leap™ tandem dyes for expanding multiplexing
• New antibody clones and dye conjugates continuously in development

View our full catalog of Biotium Choice antibodies