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Chemiluminescent Western Blot Kit

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Katalog-Nummer orb342274

Size : 1Kit

Marke : Biorbyt

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    Chemiluminescent Western Blot Kit

    Catalog Number: orb342274

    Catalog Numberorb342274
    CategoryAssays and Kits
    DescriptionChemiluminescent Western Blot Kit
    Tested applicationsWB
    Concentrationn/a
    Dilution rangeWB: User Optimized
    Form/Appearancen/a
    PurityThis Chemiluminescent Western Blot Kit allows for the detection of FLAG -tagged recombinant protein present in cell lysates provided by the user.
    ConjugationUnconjugated
    Hazard InformationNon-Toxic
    StorageSee kit insert for complete instructions.
    Buffer/PreservativesWash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!
    Alternative namesFlag Western Blot, Western Blotting Kit, Chemilumi
    NoteFor research use only
    Application notesUse Rockland Immuno-chemicals’ Anti-DYKDDDDK (FLAG) Chemiluminescent Kit for Western Blotting for detection of FLAG -tagged recombinant proteins by Western blot. This kit is useful for both ''Western blotting'' and ''dot blotting'' methods. After protein separation and transfer, the membrane is probed with Anti-DYKDDDDK (FLAG). Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
    Expiration DatePlease enquire.
    Chemiluminescent Western Blot Kit

    Flow Diagram for Chemiluminescent Western Blot Procedure

    Chemiluminescent Western Blot Kit

    Western Blot. Anti-DYKDDDDK (FLAG) Chemiluminescent Kit for Western blot was used to detect known amounts of bacterial alkaline phosphatase (BAP) protein containing the FLAG epitope tag. N-terminal linked and C-terminal linked BAP were diluted into a HeLa

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