CMLD-2 [958843-91-9]
Katalog-Nummer HY-124828-5mg
Size : 5mg
Marke : MedChemExpress
CMLD-2, an inhibitor of HuR-ARE interaction, competitively binds HuR protein disrupting its interaction with adenine-uridine rich elements (ARE)-containing mRNAs (Ki=350 nM). CMLD-2 induces apoptosis exhibits antitumor activity in different cancer cells as colon, pancreatic, thyroid and lung cancer cell lines. Hu antigen R (HuR) is an RNA binding protein, can regulate target mRNAs stability and translation.
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CMLD-2 Chemische Struktur
CAS. Nr. : 958843-91-9
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Beschreibung |
CMLD-2, an inhibitor of HuR-ARE interaction, competitively binds HuR protein disrupting its interaction with adenine-uridine rich elements (ARE)-containing mRNAs (Ki=350 nM). CMLD-2 induces apoptosis exhibits antitumor activity in different cancer cells as colon, pancreatic, thyroid and lung cancer cell lines. Hu antigen R (HuR) is an RNA binding protein, can regulate target mRNAs stability and translation[1][2]. |
IC50 & Target |
HuR[1] |
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In Vitro |
CMLD-2 (1-75 μM; 24-72 h) inhibits thyroid cancer cell viability[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability Assay[2]
Apoptosis Analysis[3]
Cell Cycle Analysis[3]
Western Blot Analysis[3]
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Molekulargewicht |
513.58 |
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Formel |
C31H31NO6 |
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CAS. Nr. |
958843-91-9 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
O=C1C=C(C2=CC=CC=C2)C3=C(OC)C=C(OC)C(C(C4=CC=C(OC)C=C4)CC(N5CCCC5)=O)=C3O1 |
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Versand | Room temperature in continental US; may vary elsewhere. |
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Speicherung |
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Lösungsmittel & Löslichkeit |
In Vitro:
DMSO : 50 mg/mL (97.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Reinheit & Dokumentation |
Purity: 98.99% |
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Verweise |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 1.9471 mL | 9.7356 mL | 19.4712 mL | 48.6779 mL |
5 mM | 0.3894 mL | 1.9471 mL | 3.8942 mL | 9.7356 mL | |
10 mM | 0.1947 mL | 0.9736 mL | 1.9471 mL | 4.8678 mL | |
15 mM | 0.1298 mL | 0.6490 mL | 1.2981 mL | 3.2452 mL | |
20 mM | 0.0974 mL | 0.4868 mL | 0.9736 mL | 2.4339 mL | |
25 mM | 0.0779 mL | 0.3894 mL | 0.7788 mL | 1.9471 mL | |
30 mM | 0.0649 mL | 0.3245 mL | 0.6490 mL | 1.6226 mL | |
40 mM | 0.0487 mL | 0.2434 mL | 0.4868 mL | 1.2169 mL | |
50 mM | 0.0389 mL | 0.1947 mL | 0.3894 mL | 0.9736 mL | |
60 mM | 0.0325 mL | 0.1623 mL | 0.3245 mL | 0.8113 mL | |
80 mM | 0.0243 mL | 0.1217 mL | 0.2434 mL | 0.6085 mL |