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> COVID-19 Spike-ACE2 Binding Assay Kit

COVID-19 Spike-ACE2 Binding Assay Kit

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Katalog-Nummer CoV-SACE2-1

Size : 1PlateKit

Marke : RayBiotech


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Figure adapted from Phan et al., Biomacromolecules, 2023.The kit was used to measure concentration-dependent reduction of recombinant SARS-CoV-2 spike RBD binding to human ACE2 in an ELISA competition assay. Figure adapted from Beeg et al., International Journal of Molecular Sciences, 2023.Figure adapted from Amruta et al., PLOS ONE, 2023.The COVID-19 Spike–ACE2 Binding Assay Kit was used to measure inhibition of SARS-CoV-2 spike receptor-binding domain (RBD) interaction with human ACE2 in the presence of bazedoxifene. Figure adapted from Omotuyi et al., Microbial Pathogenesis, 2023.The kit was used to assess changes in SARS-CoV-2 spike receptor-binding domain interaction with human ACE2 following visible-light photocatalytic treatment. Figure adapted from Matsuyama et al., Biotechnology Letters, 2023.COVID-19 Spike-ACE2 Binding Assay Kit

Figure adapted from Phan et al., Biomacromolecules, 2023.

COVID-19 Spike-ACE2 Binding Assay Kit (ACE2 on plate + Spike RBD query)

The RayBio® COVID-19 Spike-ACE2 binding assay kit is a patented, rapid, simple, and sensitive method to characterize the binding affinity of the SARS-CoV-2 Spike (S) protein and the Angiotensin I Converting Enzyme 2 (ACE2) receptor complex in the presence of potential inhibitors.

Positive and negative controls for the Spike-ACE2 binding assays are available as an accessory item. Click here.

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Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman, SARS-CoV-2
Compatible Sample TypesPlasma, Serum, Antibodies, Proteins, Small Molecules, Peptides
Accession Number
QHD43416
Gene Symbols
ACE2, S, RBD, SRBD, S-RBD
Protein Name / Synonyms
Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15, Processed angiotensin-converting enzyme 2, ACE2, Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD)
Format
ACE2 on plate + Spike RBD query
Quantitative/Semi-QuantitativeSemi-Quantitative
Solid Support
96-well Microplate
Method Of DetectionColorimetric
Research AreaProtein Therapeutics, Receptor-Ligand Binding, Infectious Disease, COVID-19
Shipping TypeBlue ice
Storage4°C
Note
The binding assay comes in two different options based on which protein is immobilized on the plate and which protein is added in solution (i.e. "query"). It is the researcher's responsibility to select the format for their study.

Product Features

  • Evaluate inhibitor activity
  • Screen inhibitors of the SARS-CoV-2 Spike-ACE2 complex
  • Characterize antibody function
  • Vaccine development
  • Selection of patient serum for antibody therapy

Validation Data

Inhibition of Spike-ACE2 by anti-viral compounds
Inhibition of Spike-ACE2 by anti-viral compounds
Four small molecules tested for neutralizing activity with COVID-19 Spike-ACE2 Binding Assay (COV-SACE2).
Inhibition of Spike-ACE2 by anti-Spike antibodies
Inhibition of Spike-ACE2 by anti-Spike antibodies
Four antibodies tested for neutralizing activity with COVID-19 Spike-ACE2 Binding Assay (COV-SACE2). nAb 1 = 130-10807; nAb 2 = 130-10844; nAb 3 = 130-10845; negative control = 130-10813.

Typical Data

The COVID-19 Spike–ACE2 Binding Assay Kit was used to measure inhibition of recombinant SARS-CoV-2 spike RBD binding to hACE2, showing dose-dependent suppression of the spike–ACE2 interaction by biomimetic spike protein nanoparticles. Figure adapted from Phan et al., Biomacromolecules, 2023.

The COVID-19 Spike–ACE2 Binding Assay Kit was used to measure concentration-dependent reduction of recombinant SARS-CoV-2 spike RBD binding to human ACE2 in an ELISA competition assay. Figure adapted from Beeg et al., International Journal of Molecular Sciences, 2023.

The COVID-19 Spike–ACE2 Binding Assay Kit was used to measure inhibition of SARS-CoV-2 spike RBD binding to human ACE2 following acetic acid treatment, demonstrating reduced spike–ACE2 interaction after viral protein exposure to acetic acid. Figure adapted from Amruta et al., PLOS ONE, 2023.

The COVID-19 Spike–ACE2 Binding Assay Kit was used to measure inhibition of SARS-CoV-2 spike receptor-binding domain (RBD) interaction with human ACE2 in the presence of bazedoxifene. Figure adapted from Omotuyi et al., Microbial Pathogenesis, 2023.

The COVID-19 Spike–ACE2 Binding Assay Kit was used to assess changes in SARS-CoV-2 spike receptor-binding domain interaction with human ACE2 following visible-light photocatalytic treatment. Figure adapted from Matsuyama et al., Biotechnology Letters, 2023.

Storage/Stability

The kit may be stored at 4°C up to 1 month from the date of shipment. The life of the kit can be extended up to 6 months from the date of shipment by storing the kit reagents (Item F, C, and D) at -20°C. All other components should be stored at 4°C.

citations

  • A single mutation may contribute to accelerated evolution of SARS-CoV-2 toward Omicron

    Sample Type:
    Purified / Recombinant Protein (recombinant RBD proteins)
    Year:
    2025
    Journal Name:
    Nature Communications
    PubMed ID:
    40730568

  • PoMA-10: a dual-action antiviral disrupting SARS-CoV-2 Spike-ACE2 interaction and protecting lung tissue

    Sample Type:
    Other / Unspecified (candidate Spike-ACE2 blocker compounds)
    Year:
    2026
    Journal Name:
    Frontiers in Pharmacology
    PubMed ID:
    41908829

  • Characterization of Androctonus mauritanicus venom and in vitro screening of SARS-CoV-2 entry inhibitors candidates

    Species:
    Other
    Sample Type:
    Other / Unspecified (purified fractions of the venom)
    Year:
    2025
    Journal Name:
    Frontiers in Pharmacology
    PubMed ID:
    41256256

  • Nanostructured biohybrid material with wide-ranging antiviral action

    Year:
    2023
    Journal Name:
    Nano Research

  • Assessment of the effect of Nowarta110 on the interaction between the SARS-CoV-2 spike protein and ACE2

    Sample Type:
    Other / Unspecified (Nowarta110 dilutions)
    Year:
    2025
    Journal Name:
    International Journal of Herbal Medicine

  • Drug repurposing of argatroban, glimepiride and ranolazine shows anti-SARS-CoV-2 activity via diverse mechanisms

    Sample Type:
    Other / Unspecified (shortlisted FDA-approved drugs cell-free binding assay)
    Year:
    2025
    Journal Name:
    Heliyon
    PubMed ID:
    39968139

  • GUNDELIA CAPPADOCICA BİTKİSİNİN KİMYASAL KOMPOZİSYONU, COVID-19 SPIKE ACE2 BAĞLANMA İNHİBİSYON POTANSİYELİ İLE IN SILICO MOLECULAR DOCKING VE ADME ÖZELLİKLERİNİN BELİRLENMESİ

    Sample Type:
    Other / Unspecified (ethanolic extract of gundelia cappadocica)
    Year:
    2025
    Journal Name:
    Doğu Karadeniz Sağlık Bilimleri Dergisi

  • Phytochemical Profiling and in Vitro Anti-Sars-Cov-2 Evaluation of Verbascum Zerdust: Integration of Ace2-Spike Inhibition Assay and Computational Approaches

    Sample Type:
    Other / Unspecified (ethanolic extract of V. zerdust)
    Year:
    2025
    Journal Name:
    Bitlis Eren University Journal of Science and Technology

  • 香辛料由来マラバリコーンCによるSARS-CoV-2感染阻害とその機構解明

    Sample Type:
    Other / Unspecified (assayed compounds cell-free Spike-ACE2 binding)
    Year:
    2025
    Journal Name:
    Institutional Repositories DataBase (IRDB)

  • ACE2- and HR2-Mimetic Peptides Inhibit Replication of Two SARS-CoV-2 Variants

    Sample Type:
    Other / Unspecified (designed antiviral peptides P-Nat, P-3)
    Year:
    2026
    Journal Name:
    Journal of Medical Virology
    PubMed ID:
    42473348

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