Cytokeratin 18 (KRT18)(B23.1), 1mg/mL
Katalog-Nummer BNUM1263-50
Size : 50uL
Marke : Biotium
Cytokeratin 18 (KRT18)(B23.1), 1mg/mL
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Considerations for Immunofluorescence Staining
Primary & Secondary Antibody Conjugates
Cytokeratin 18 Monoclonal Mouse Antibody (B23.1)
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Product Description
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Catalog number key for antibody number 1263, Anti-Cytokeratin 18 (B23.1)
| Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
|---|---|---|---|---|---|
| BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
| BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
| BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
| BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
| BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
| BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
| BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
| BNCB | Biotin | N/A | N/A | N/A | |
| BNUB | Purified | N/A | N/A | N/A | |
| BNUM | Purified, BSA-free | N/A | N/A | N/A |
Technology
Product Attributes
Antibody number
#1263
Antibody reactivity (target)
Cytokeratin 18
Antibody type
Primary
Host species
Mouse
Clonality
Monoclonal
Clone
B23.1
Isotype
IgG1, kappa
Molecular weight
45 kDa
Synonyms
Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18
Human gene symbol
KRT18
Entrez gene ID
3875
SwissProt
P05783
Unigene
406013
Immunogen
PMC-42 human breast carcinoma cells
Verified antibody applications
IHC (FFPE) (verified), WB (verified)
Antibody target cellular localization
Cytoskeleton
Species reactivity
Human
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 1-2 ug/mL, Immunohistochemistry (formalin-fixed): 0.5-1 ug/mL for 30 min at RT, Flow cytometry: 0.5-1 ug/million cells, Western Blot 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user
Positive control
HCT116, MCF-7, HeLa, A431 cells. Breast Cancer.
Shipping condition
Room temperature
Storage Conditions
Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Regulatory status
For research use only (RUO)
Antibody/conjugate formulation
Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Shelf life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Cell/tissue expression
Epithelial cells
Product origin
Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Antibody research areas
Cancer, Cytoskeleton
Documents, Protocols, SDS and COA
Datasheet
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