Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie
 
> DGDG [63142-69-8]

DGDG [63142-69-8]

Drucken Drucken
Melden Sie sich an

Katalog-Nummer HY-143693-1mg

Size : 1mg

Marke : MedChemExpress


Beschreibung

DGDG, a chloroplast lipid, is a bilayer-forming lipid. DGDG is important for photosynthesis, and can be used for drug delivery[1].

Cellular Effect
Cell Line Type Value Description References
Vero CC50
> 100 μg/mL
Compound: 1
Cytotoxicity against african green monkey Vero cells after 72 hrs by resazurin dye-based fluorimetric analysis
Cytotoxicity against african green monkey Vero cells after 72 hrs by resazurin dye-based fluorimetric analysis
[PMID: 22348826]
In Vitro

Apart from facilitating the photosynthesis light reaction in the thylakoid membrane, DGDG is important for maintaining chloroplast morphology and for plant survival under abiotic stresses such as phosphate starvation and freezing. During phosphate limitation, the amounts of DGDG increase in the plastids of plants, and DGDG is exported to extraplastidial membranes to replace phospholipids[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DGDG Related Antibodies

Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 100 mg/mL (106.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0670 mL 5.3350 mL 10.6701 mL
5 mM 0.2134 mL 1.0670 mL 2.1340 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (2.67 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (2.67 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Reinheit & Dokumentation
Verweise