DNase I (GMP-grade)

DNase I is a deoxyribonucleic acid endonuclease that hydrolyzes single- or double-stranded DNA, producing dinucleotide, trinucleotide, and oligonucleotide products with 3’-hydroxyl and 5’-phosphate group ends. The activity of DNase I is Ca²⁺ dependent and can be activated by either Mg²⁺ or Mn²⁺. In the presence of Mg²⁺, DNase I can randomly cleave any site of double-stranded DNA. In the presence of Mn²⁺, DNase I recognizes both strands of DNA simultaneously and cleaves them at nearly identical sites.

DNase I can act on single-stranded DNA, double-stranded DNA, chromatin, and RNA:DNA hybrids. It is suitable for RNA extraction, in vitro transcription, and DNA removal in RT-PCR experiments. The production process of this product strictly controls host protein residues, nucleic acid residues, etc., and conforms to GMP production and quality management standards.