Estrogen Receptor, beta (ES) (AP)
Katalog-Nummer MBS6122249-0,1mL
Size : 0.1mL
Marke : MyBiosource
Estrogen Receptor, beta (ES) (AP)
Formalin-fixed, paraffin-embedded breast cancer stained with Estrogen receptor beta ERb455 antibody at 1:200 using peroxidase-conjugate and DAB chromogen.
Formalin-fixed, paraffin-embedded ovarian cancer stained with Estrogen receptor beta ERb455 antibody at 1:200 using peroxidase-conjugate and DAB chromogen.
Estrogen Receptor, Beta (ES) Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6122249MBS6122249 is an antibody targeting ES. Also known as Estrogen Receptor, beta (ES) (AP). Reactive in Equine, Human, Monkey, Mouse, Porcine, Rat, Sheep. Host: Mouse. More details
Product Overview
Online Datasheet in PDFProduct Name
Estrogen Receptor, beta, Monoclonal Antibody
Full Product Name
Estrogen Receptor, beta (ES) (AP)
Product Gene Name
anti-ES antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
ERb455
Host
Mouse
Species Reactivity
Equine, Human, Monkey, Mouse, Porcine, Rat, Sheep
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein G Affinity Chromatography
Specificity
Recognizes human Estrogen Receptor beta. Species Crossreactivity: monkey, mouse, rat, porcine, equine and sheep.
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Molecular Weight
53,209 Da
OMIM
601663
Immunogen
Recombinant protein corresponding to human Estrogen Receptor beta at C-terminus.
Conjugate
AP
Positive Control
Ovarian, breast or prostate carcinoma
Product Categories/Family for anti-ES antibody
Antibodies; Receptors; Estrogen
Applications Tested/Suitable for anti-ES antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-ES antibody
IP: 1-2ug/500ug protein lysate
WB: 0.5-1ug/ml
Applications are based on unconjugated antibody.
WB: 0.5-1ug/ml
Applications are based on unconjugated antibody.
Product References and Citations for anti-ES antibody
1. Skliris GP et. al. J Pathol 2002;197:155-62.
Other Notes
Small volumes of anti-ES antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for ES. It may not necessarily be applicable to this product.
NCBI Official Full Name
estrogen receptor beta isoform 2
NCBI Official Synonym Full Names
estrogen receptor 2 (ER beta)
NCBI Official Symbol
ESR2
NCBI Official Synonym Symbols
Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
UniProt Protein Name
Estrogen receptor beta
UniProt Synonym Protein Names
Nuclear receptor subfamily 3 group A member 2
Protein Family
Estrogen receptor
UniProt Gene Name
ESR2
UniProt Synonym Gene Names
ESTRB; NR3A2; ER-beta
UniProt Entry Name
ESR2_HUMAN
NCBI Protein Information
estrogen receptor beta; estrogen receptor beta 4; nuclear receptor subfamily 3 group A member 2
UniProt Comments for ES
ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.
Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 14q23.2
Cellular Component: nucleoplasm; mitochondrion; extracellular region; nucleus
Molecular Function: ligand-dependent nuclear receptor activity; receptor antagonist activity; protein binding; estrogen receptor activity; enzyme binding; DNA binding; zinc ion binding; transcription coactivator activity; estrogen response element binding; steroid hormone receptor activity; transcription factor activity; steroid binding
Biological Process: transcription initiation from RNA polymerase II promoter; estrogen receptor signaling pathway; induction of apoptosis by hormones; neuron migration; vagina development; negative regulation of transcription from RNA polymerase II promoter; uterus development; signal transduction; cell-cell signaling; ovarian follicle development; regulation of transcription, DNA-dependent; steroid hormone mediated signaling; positive regulation of transcription factor activity; gene expression; brain development; negative regulation of cell growth; negative regulation of epithelial cell proliferation
Show more Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 14q23.2
Cellular Component: nucleoplasm; mitochondrion; extracellular region; nucleus
Molecular Function: ligand-dependent nuclear receptor activity; receptor antagonist activity; protein binding; estrogen receptor activity; enzyme binding; DNA binding; zinc ion binding; transcription coactivator activity; estrogen response element binding; steroid hormone receptor activity; transcription factor activity; steroid binding
Biological Process: transcription initiation from RNA polymerase II promoter; estrogen receptor signaling pathway; induction of apoptosis by hormones; neuron migration; vagina development; negative regulation of transcription from RNA polymerase II promoter; uterus development; signal transduction; cell-cell signaling; ovarian follicle development; regulation of transcription, DNA-dependent; steroid hormone mediated signaling; positive regulation of transcription factor activity; gene expression; brain development; negative regulation of cell growth; negative regulation of epithelial cell proliferation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-ES antibody
| Reference Product | PubMed Publications |
|---|---|
| ESR1 antibody | >235 publications with ES and ESR1 |
| NRIP1 antibody | >6 publications with ES and NRIP1 |
| SRC antibody | >3 publications with ES and SRC |
| OXT antibody | >2 publications with ES and OXT |
| NCOA3 antibody | >2 publications with ES and NCOA3 |
| NCOA1 antibody | >2 publications with ES and NCOA1 |
| STAT5A antibody | >1 publications with ES and STAT5A |
| SP1 antibody | >1 publications with ES and SP1 |
| NCOR2 antibody | >1 publications with ES and NCOR2 |
| AKT1 antibody | >1 publications with ES and AKT1 |
Pathways associated with anti-ES antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Estrogen Signaling Pathway antibodies | Estrogen Signaling Pathway Diagram |
| Estrogen Signaling Pathway antibodies | Estrogen Signaling Pathway Diagram |
| Gene Expression Pathway antibodies | Gene Expression Pathway Diagram |
| Generic Transcription Pathway antibodies | Generic Transcription Pathway Diagram |
| Integrated Pancreatic Cancer Pathway antibodies | Integrated Pancreatic Cancer Pathway Diagram |
| Nuclear Receptor Transcription Pathway antibodies | Nuclear Receptor Transcription Pathway Diagram |
| Nuclear Receptors Pathway antibodies | Nuclear Receptors Pathway Diagram |
| Ovarian Infertility Genes Pathway antibodies | Ovarian Infertility Genes Pathway Diagram |
| Plasma Membrane Estrogen Receptor Signaling Pathway antibodies | Plasma Membrane Estrogen Receptor Signaling Pathway Diagram |
| Prolactin Signaling Pathway antibodies | Prolactin Signaling Pathway Diagram |
Diseases associated with anti-ES antibody
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >73 publications with ES and Neoplasms |
| Cardiovascular Diseases Antibodies | >27 publications with ES and Cardiovascular Diseases |
| Breast Neoplasms Antibodies | >25 publications with ES and Breast Neoplasms |
| Genital Diseases, Male Antibodies | >21 publications with ES and Genital Diseases, Male |
| Colorectal Neoplasms Antibodies | >13 publications with ES and Colorectal Neoplasms |
| Endometriosis Antibodies | >11 publications with ES and Endometriosis |
| Infertility, Female Antibodies | >11 publications with ES and Infertility, Female |
| Hypertension Antibodies | >10 publications with ES and Hypertension |
| Carcinoma Antibodies | >10 publications with ES and Carcinoma |
| Infertility, Male Antibodies | >8 publications with ES and Infertility, Male |
Organs/Tissues associated with anti-ES antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Testis Antibodies | >19 publications with ES and Testis |
| Embryonic Tissue Antibodies | >4 publications with ES and Embryonic Tissue |