Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Etomoxir [124083-20-1]

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Katalog-Nummer NB-64-28792-5mg

Size : 5mg

Marke : Neo Biotech

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Etomoxir

(Synonyms: (R)-(+)-Etomoxir) Copy Product Info

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Synonyms: (R)-(+)-Etomoxir

Catalog No. T4535L Copy Product Info

Purity: 99.47%

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Etomoxir is an irreversible inhibitor of carnitine palmitoyltransferase 1a (CPT-1a) (IC50=5-20 nM). Etomoxir inhibits fatty acid oxidation by inhibiting CPT-1a, inhibits palmitate oxidation, has an inhibitory effect on adenine nucleotide translocase, and can inhibit macrophage polarization by disrupting CoA homeostasis.

Cas No. 124083-20-1

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.47%

Appearance:Solid

Color:White

Product Introduction

Bioactivity

Description	Etomoxir is an irreversible inhibitor of carnitine palmitoyltransferase 1a (CPT-1a) (IC50=5-20 nM). Etomoxir inhibits fatty acid oxidation by inhibiting CPT-1a, inhibits palmitate oxidation, has an inhibitory effect on adenine nucleotide translocase, and can inhibit macrophage polarization by disrupting CoA homeostasis.
Targets & IC50	KB cells (human):2.76 μM, faTT cellsy acid (Hepatocyte cells,human):0.1 μM, faTT cellsy acid (Hepatocyte cells,guinea pig):1 μM, faTT cellsy acid (Hepatocyte cells,rat):10 μM
In vitro	METHODS: A2780/PTX cells were treated with Etomoxir (1, 10, 100, 1000, 10000, 100000 nM) and paclitaxel (1, 10, 100, 1000, 10000, 100000 nM) for 48 hours, and the growth inhibition of the cells was detected by the CCK-8 method. RESULTS: The IC50 value of Etomoxir decreased from 1589.5 ± 62.5 nM to 817.4 ± 79.7 nM. [1]
In vivo	METHODS: To study the inhibition of cardiac CPT-I activity by Etomoxir, rats were injected with Etomoxir daily for 8 consecutive days at a dose of 20 mg/kg. RESULTS: Rats treated with Etomoxir showed a 44% reduction in cardiac CPT-I activity. METHODS: To study the effects of Etomoxir on blood glucose levels, weight gain, hind limb muscle mass, heart mass and liver mass, Lewis rats were treated with 20 mg/kgEtomoxir for 8 days. RESULTS: Treating Lewis rats with Etomoxir for 8 days did not change the blood glucose level, did not affect general growth characteristics such as weight gain, nor did it affect the muscle mass of the hind limbs. However, both the heart mass and liver mass increased significantly by 11%. [2]
Synonyms	(R)-(+)-Etomoxir

Chemical Properties

Molecular Weight	326.82
Formula	C₁₇H₂₃ClO₄
Cas No.	124083-20-1
Smiles	C(OCC)(=O)[C@@]1(CCCCCCOC2=CC=C(Cl)C=C2)CO1
Relative Density.	1.163 g/cm3

Storage & Solubility Information

Storage

Store at low temperature,Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 250 mg/mL (764.95 mM), Sonication and heating are recommended.

In Vivo Formulation

0.5% CMC-Na: 12.5 mg/mL (38.25 mM), Suspension.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.0598 mL	15.2989 mL	30.5979 mL	152.9894 mL
5 mM	0.6120 mL	3.0598 mL	6.1196 mL	30.5979 mL
10 mM	0.3060 mL	1.5299 mL	3.0598 mL	15.2989 mL
20 mM	0.1530 mL	0.7649 mL	1.5299 mL	7.6495 mL
50 mM	0.0612 mL	0.3060 mL	0.6120 mL	3.0598 mL
100 mM	0.0306 mL	0.1530 mL	0.3060 mL	1.5299 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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Keywords

EtomoxirCPT1Antioxidant

Related Tags: Etomoxir chemical structure | Etomoxir in vivo | Etomoxir in vitro | Etomoxir formula | Etomoxir molecular weight