Size : 10mg
Synonyms: FITC-Dextran (60842-46-8 MW 4000)
| Description | FITC-Dextran (MW 4000) is a fluorescein isothiocyanate (FITC)-labeled dextran fluorescent probe (Ex=495 nm; Em=525 nm) that combines the biocompatibility and hydrophilicity of dextran with the green fluorescence properties of FITC. FITC-Dextran (MW 4000) serves as a marker to reveal heat shock-induced cellular damage and investigate early and late stages of apoptosis. It is also applicable for studies on cellular permeability, such as blood-brain barrier permeability and assessment of blood-brain barrier disruption. |
| Synonyms | FITC-Dextran (60842-46-8 MW 4000) |
| Molecular Weight | 4000 (Average) |
| Storage | Keep away from direct sunlight,Keep away from moisture,Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | H2O: 80 mg/mL, Sonication is recommended. DMSO: 10 mg/mL, Sonication is recommended. |
| In Vivo Formulation | PBS: 100 mg/mL, Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. |
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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