Free Fatty Acids (FFA) Fluorometric Assay Kit

Intended Uses: This kit can be used to measure the non-esterified free fatty acids (FFA) content in serum, plasma, animal tissue samples.

Principle of the Assay: Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.

Background/Introduction: Free fatty acids, also known as non-esterified fatty acids, are derived from dietary or the metabolism of adipose tissue. In adipose tissue, hormonesensitive lipase (HSL) decomposes triglycerides to produce glyc erol and fatty acids. Circulating in the body with free fatty acids combined with plasma albumin, used as an energy source easily absorbed by muscles, brains, and other tissues and organs. FFA is not only the product of fat hydrolysis, but also the substrate of fat synthesis. The concentration of FFA is related to lipid metabolism, glucose metabolism and endocrine function.

Small volumes of FFA assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2567669 is an assay kit targeting FFA. Also known as Free Fatty Acids (FFA) Fluorometric Assay Kit.