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General Block ELISA Blocking Buffer

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Katalog-Nummer orb1821225-100ml

Size : 100ml

Marke : Biorbyt


General Block ELISA Blocking Buffer

SKU: orb1821225

Description

Contains a mammalian protein blocking agent suitable for most antibody capture ELISA formats and peptide/protein antigen-down ELISA formats. It provides a stable long-term environment for dried antigen or antibody and minimizes non-specific binding.

Images & Validation

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General Block ELISA Blocking Buffer
Application Notes
1. Coat antibody or antigen onto the ELISA plate using ICT’s Antibody Coating Buffer or Antigen Coating Buffer. 2. Incubate 8–24 hours at room temperature (RT). 3. Aspirate the coating solution. 4. Wash each well twice with ICT’s ELISA Wash Buffer. 5. Block the uncoated regions of the ELISA plate by pipetting 300–400 µL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution. 6. Incubate 8–24 hours at RT. For best blocking, incubate overnight at RT. 7. Aspirate the blocking buffer; do not wash. 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.

Key Properties

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Storage & Handling

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Storage2-8°C
Concentration1 X
Expiration Date12 months from date of receipt.
DisclaimerFor research use only
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