Gja1 (NM_010288) Mouse Untagged Clone
Katalog-Nummer MC205621
Size : 10ug
Gja1 (NM_010288) Mouse Untagged Clone
SKU
MC205621
Gja1 (untagged) - Mouse gap junction protein, alpha 1 (Gja1), (10ug)
| Product Data | |
| Type | Mouse Untagged Clone |
|---|---|
| Target Symbol | Gja1 |
| Synonyms | AU042049; AW546267; Cnx43; connexin43; Cx43; Cx43alpha1; Gja-1; Npm1 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | RsrII-NotI | |
| ACCN | NM_010288 |
| Insert Size | 1149 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@clinisciences.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC006894, AAH06894 |
| RefSeq Size | 3071 bp |
| RefSeq ORF | 1149 bp |
| Locus ID | 14609 |
| UniProt ID | P23242 |
| Cytogenetics | 10 28.64 cM |
| Summary | Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. May play a role in cell growth inhibition through the regulation of NOV expression and localization (PubMed:15181016). Plays an essential role in gap junction communication in the ventricles (PubMed:26403541).UniProtKB/Swiss-Prot Function |
| Product Manuals |
| FAQs |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| MG205964 | Gja1 (tGFP-tagged) - Mouse gap junction membrane channel protein alpha 1 (Gja1) | 10 ug | | |
| MR205964 | Gja1 (Myc-DDK-tagged) - Mouse gap junction protein, alpha 1 (Gja1) | 10 ug | | |
| MR205964L1 | Lenti ORF clone of Gja1 (Myc-DDK-tagged) - Mouse gap junction protein, alpha 1 (Gja1) | 10 ug | | |
| MR205964L2 | Lenti ORF clone of Gja1 (mGFP-tagged) - Mouse gap junction protein, alpha 1 (Gja1) | 10 ug | | |
| MR205964L3 | Lenti ORF clone of Gja1 (Myc-DDK-tagged) - Mouse gap junction protein, alpha 1 (Gja1) | 10 ug | | |
| MR205964L4 | Lenti ORF clone of Gja1 (mGFP-tagged) - Mouse gap junction protein, alpha 1 (Gja1) | 10 ug | | |
Citations
20 kDa isoform of connexin-43 augments spatial reorganization of the brain endothelial junctional complex and lesion leakage in cerebral cavernous malformation type-3
Phillips, Johnson, Stamatovic et al
Neurobiol Dis (2023) 186, 106277 DOI: 10.1016/j.nbd.2023.106277
Phillips, Johnson, Stamatovic et al
Neurobiol Dis (2023) 186, 106277 DOI: 10.1016/j.nbd.2023.106277
Cited by 10
AI Summary: OriGene’s murine Cx43 cDNA clone (Cat# MC205621) was used as a template to generate full-length and 20 kDa isoform Cx43 constructs, enabling the study of their roles in endothelial junction organization and vascular leakage in cerebral cavernous malformation type-3.
Snippet: The following Cx43 constructs were generated using the murine Cx43 sequence from cDNA clone (OriGene, Cat# MC205621) as a template: full length connexin-43 (Cx43-FL, aa1–382), Cx43 20 kDa isoform with methionine (M) start codon at 213 (GJA1–20 k
TLR2-Dependent Reversible Oxidation of Connexin 43 at Cys260 Modifies Electrical Coupling After Experimental Myocardial Ischemia/Reperfusion
Raimann, Dröse, Bonke et al
J Cardiovasc Transl Res (2019)
Raimann, Dröse, Bonke et al
J Cardiovasc Transl Res (2019)
AI Summary: OriGene’s pCMV6-Kan/Neo-Gja1ORF plasmid (MC205621) was used as the template to amplify and generate wild-type and mutant Connexin 43 constructs for functional studies in this paper.
Snippet: Full-length protein-coding regions of mmGja1 and mmGja1-C260A were PCR amplified with Fusion High-Fidelity DNA Polymerase (F530L, Thermo Fisher) from pCMV6-Kan/Neo-Gja1ORF (MC205621, Origene) with the primers 5′-ATTTGCGGCCGCA
Loss of Elp3 Impairs the Acetylation and Distribution of Connexin-43 in the Developing Cerebral Cortex
Laguesse, Close, Van Hees et al
Front Cell Neurosci (2017) 11, 122 DOI: 10.3389/fncel.2017.00122
Laguesse, Close, Van Hees et al
Front Cell Neurosci (2017) 11, 122 DOI: 10.3389/fncel.2017.00122
AI Summary: OriGene’s Cx43 clone (catalog number MC205621) was used as the source for subcloning Connexin-43, enabling the study of its acetylation and distribution in developing cerebral cortex.
Snippet: All constructs were sequence verified. Cx43 was subcloned from the clone image MC205621 (Origene, Rockville, MD, USA) by high fidelity PCR using NheI-Cx43 forward primer and EcoRV-reverse primer and inserted into the pCAGGS-IRES-GFP vector
Gap junctions/hemichannels modulate interkinetic nuclear migration in the forebrain precursors
Liu, Hashimoto-Torii, Torii et al
J Neurosci (2010) 30 (12), 4197-209 DOI: 10.1523/JNEUROSCI.4187-09.2010
Liu, Hashimoto-Torii, Torii et al
J Neurosci (2010) 30 (12), 4197-209 DOI: 10.1523/JNEUROSCI.4187-09.2010
AI Summary: OriGene’s Cx43 shRNA (sc-36200-SH) was used in this study to mediate knockdown of connexin 43, enabling the authors to investigate its role in regulating interkinetic nuclear migration in forebrain precursors.
Showing 1-4 of 4 papers.
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