Glucose-6-Phosphate Assay Kit (Colorimetric)
Katalog-Nummer MA-G6P-1
Size : 1PlateKit
Marke : RayBiotech
Glucose-6-Phosphate Assay Kit (Colorimetric)
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
|---|---|
| Estimated Lead Time | 1-2 business days |
| Sensitivity | 0.71 µM |
| Detection Range | 3.125 µM - 200 µM |
| Compatible Sample Types | Plasma, Serum, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Method Of Detection | Colorimetric |
| Research Area | Disulfidptosis, Energy Metabolism, Glycolysis & Lipid Metabolism |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Glucose 6-phosphate (G6P) is a key metabolic intermediate for glucose to enter either metabolic pathways or storage. G6P is generated when glucose is phosphorylated by hexokinase / glucokinase, or by the conversion of glucose-1-phosphate by phosphoglucomutase during glycogenolysis. G6P lies at the beginning of both glycolysis and the pentose phosphate pathways. In addition to these metabolic pathways, glucose 6-phosphate may also be converted to glycogen or starch for storage. G6P is utilized by glucose-6-phosphate dehydrogenase (G6PDH) to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.
Glucose-6-Phosphate Assay Kit provides a simple, reproducible, and sensitive tool of measuring glucose-6-phosphate concentration in plasma, serum, cell lysates, and other biological liquid samples. In this assay, glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase, the formed NADPH is coupled to the colorimetric probe that produces a colored product which can be measured at 450 nm. The optical density measured is directly proportional to the concentration of glucose-6-phosphate present in the sample.
Kit Components
| Component | Size / Description |
|---|---|
| Microplate | A 96-well plate |
| Assay Buffer | 15 mL |
| Glucose-6-Phosphate Standard | 1 vial |
| Glucose-6-Phosphate Enzyme | 1 vial |
| Coupling Reagent | 1 vial |
| Cofactor | 1 vial |
| Colorimetric Probe | 1 vial |
Additional Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Tubes to prepare sample dilutions
- 50 mL conical tubes
- MilliQ water or other type of double distilled water (ddH2O)
Assay Procedure Summary
- Prepare the Reaction Mix, and Background Mix before use.
- Add the diluted Glucose-6-Phosphate Standards or samples to the 96-well microtiter plate.
- Initiate the reaction by adding Reaction Mix to Standards and Sample wells, and Background Mix to Sample Background wells.
- Carefully shake the plate for a few seconds to mix.
- Incubate the plate for 30 minutes at room temperature with mixing in the dark.
- Measure the absorbance at 450nm using a plate reader.
