Glucose Oxidase Activity (GOD) Assay Kit

Principle of the Assay: CheKine™ Glucose Oxidase Activity Assay Kit provides a simple and easy colorimetric assay for detecting Glucose Oxidase Activity in biological samples such as in serum or plasma, cells, urine, other body fluid, food, growth medium. In this assay, GOD activity is determined by a coupled enzyme assay, in which GOD oxidizes D-glucose resulting in the production of hydrogen peroxide (H2O2) that reacts with chromogen in an acid condition, produce a product that can be measured at OD 580 nm. Therefore, the glucose oxidase activity present in the sample is proportional to the signal obtained.!!Background/Introduction: The glucose oxidase enzyme (GOx) (EC 1.1.3.4) is a dimeric enzyme that catalyzes oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid. This enzyme is produced by certain species of insects, fungi, and bacteria. Glucose oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose and oxygen from beverages, food and other agricultural products. Furthermore, Glucose oxidase is commonly used in biosensors to detect glucose.

Small volumes of GOD assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9718971 is an assay kit targeting GOD. Also known as Glucose Oxidase Activity (GOD) Assay Kit.