Glutathione Reductase (GR) Activity Assay Kit
Katalog-Nummer E-BC-K099-M-96T
Size : 96T
Marke : Elabscience
For research use only.
| Detection Principle | With the reduced coenzyme (NADPH) as hydrogen donor, oxidized glutathione (GSSG) can be reduced to reduced glutathione (GSH). The decrease of NADPH absorbance can be measured at 340 nm. The activity of GR can be calculated by detecting the change of NADPH. |
| Synonyms | GR |
| Sample Type | Serum,Plasma,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Research Area | Oxidative Stress , Ferroptosis |
| Other Reagents Required | Double distilled water, Normal saline (0.9% NaCl), Deionized water |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 1.87 U/L |
| Detection Range | 1.87-150 U/L |
| Precision | inter-assay CV: 2.1-6.8% | intra-assay CV: 1.5-3.9% |
| Sample Volume | 10 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| 10% Mouse liver tissue homogenization | 2-8 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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