Product Details
Goat Anti-Rabbit IgG (H&L) Antibody DyLight™ 800 Conjugated - 611-145-002
Goat anti-Rabbit IgG Antibody DyLight™800 Conjugation, Goat anti-Rabbit IgG DyLight™ 800 Conjugated Antibody
Goat
IgG (H&L)
DyLight™ 800
Polyclonal
IgG
Target Details
Rabbit
Rabbit IgG whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. This antibody will react with heavy chains of Rabbit IgG and with light chains of most Rabbit immunoglobulins.
Application Details
WB, ELISA, IHC, Dot Blot, EMSA, IP - View References
Anti-Rabbit IgG Antibody DyLight™800 has been tested by ELISA, dot blot, and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Formulation
1.0 mg/mL
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Rabbit IgG Antibody DyLight™800 generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to DyLight™800.
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
Fornes O et al. (2023). A multimorphic mutation in IRF4 causes human autosomal dominant combined immunodeficiency. Sci Immunol.
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Supershift/Gel Shift/EMSA PubMed
Huang M et al. (2023). Bioinformatics and network pharmacology identify promotional effects and potential mechanisms of ethanol on esophageal squamous cell carcinoma and experimental validation. Toxicol Appl Pharmacol.
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WB/IB/PCA PubMed
Meng P et al. (2023). A whole transcriptome profiling analysis for antidepressant mechanism of Xiaoyaosan mediated synapse loss via BDNF/trkB/PI3K signal axis in CUMS rats. BMC Complement Med Ther.
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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Applications
WB/IB/PCA; Dot Blot Assay PubMed
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Applications
WB/IB/PCA PubMed
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WB/IB/PCA PubMed
Xu S et al. (2021). IL-6 promotes nuclear translocation of HIF-1α to aggravate chemoresistance of ovarian cancer cells. Eur J Pharmacol.
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WB/IB/PCA PubMed
Raimo S et al. (2021). Mitochondrial morphology, bioenergetics and proteomic responses in fatty acid oxidation disorders. Redox Biol.
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WB/IB/PCA PubMed
Cao Z et al. (2021). HBP1-mediated transcriptional repression of AFP inhibits hepatoma progression. J Exp Clin Cancer Res.
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WB/IB/PCA PubMed
Liu Y et al. (2021). GP73-mediated secretion of AFP and GP73 promotes proliferation and metastasis of hepatocellular carcinoma cells. Oncogenesis.
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WB/IB/PCA PubMed
Li H et al. (2021). Icaritin promotes apoptosis and inhibits proliferation by down-regulating AFP gene expression in hepatocellular carcinoma. BMC Cancer.
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WB/IB/PCA PubMed
Agulto RL et al. (2021). Autoregulatory control of microtubule binding in doublecortin-like kinase 1. Elife.
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
Lu HY et al. (2021). Mechanistic understanding of the combined immunodeficiency in complete human CARD11 deficiency. J Allergy Clin Immunol.
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WB/IB/PCA PubMed
Strait AA et al. (2021). Distinct immune microenvironment profiles of therapeutic responders emerge in combined TGFβ/PD-L1 blockade-treated squamous cell carcinoma. Commun Biol.
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WB/IB/PCA PubMed
Fung SY et al. (2021). MALT1-Dependent Cleavage of HOIL1 Modulates Canonical NF-κB Signaling and Inflammatory Responsiveness. Front Immunol.
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
Cao et al. (2019). MDM2 promotes genome instability by ubiquitinating the transcription factor HBP1. Oncogene
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
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WB/IB/PCA PubMed
Yu S et al. (2019). RSRC1 suppresses gastric cancer cell proliferation and migration by regulating PTEN expression. Mol Med Rep.
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WB/IB/PCA PubMed
Goutierre M et al. (2019). KCC2 regulates neuronal excitability and hippocampal activity via interaction with Task-3 channels. Cell Rep.
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WB/IB/PCA PubMed
Sriramachanfran AM et al. (2019). Arkadia/RNF111 is a SUMO-targeted ubiquitin ligase with preference for substrates marked with SUMO1-capped SUMO2/3 chain. Nat Commun.
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WB/IB/PCA PubMed
Miller KE et al. (2019). Kif2a scales meiotic spindle size in Hymenochirus boettgeri. Curr Biol.
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WB/IB/PCA PubMed
Wang M et al. (2019). Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization. J Vis Exp.
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WB/IB/PCA PubMed
Zhang D et al. (2019). High iodine effects on the proliferation, apoptosis, and migration of papillary thyroid carcinoma cells as a result of autophagy induced by BRAF kinase. Biomed Pharmacother.
Applications
WB/IB/PCA PubMed
Lin et al. (2018). Liver-specific deletion of Eva1a/Tmem166 aggravates acute liver injury by impairing autophagy. Cell Death & Disease
Applications
IHC/ICC/Histology; WB/IB/PCA PubMed
Zhang et al. (2018). Amelioratory Effects of Testosterone Propionate on Age-related Renal Fibrosis via Suppression of TGF-β1/Smad Signaling and Activation of Nrf2-ARE Signaling. Scientific Reports
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WB/IB/PCA PubMed
Myles et al. (2018). TNF overproduction impairs epithelial staphylococcal response in hyper IgE syndrome. Journal of Clinical Investigation
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WB/IB/PCA PubMed
Yu S et al. (2018). PPP2R2D, a regulatory subunit of protein phosphatase 2A, promotes gastric cancer growth and metastasis via mechanistic target of rapamycin activation. Int J Oncol.
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WB/IB/PCA PubMed
Wang et al. (2017). A positive feedback loop between Pim-1 kinase and HBP1 transcription factor contributes to hydrogen peroxide-induced premature senescence and apoptosis. Journal of Biological Chemistry
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WB/IB/PCA PubMed
Li et al. (2017). Deletion of Pdcd5 in mice led to the deficiency of placenta development and embryonic lethality. Cell Death & Disease
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WB/IB/PCA PubMed
Wang et al. (2017). Efficacy of Postnatal In Vivo Nonsense Suppression Therapy in a Pax6 Mouse Model of Aniridia. Molecular Therapy - Nucleic Acids
Applications
WB/IB/PCA PubMed
Xia et al. (2017). Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress. Cell Death & Disease
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WB/IB/PCA PubMed
Heubl et al. (2017). GABAA receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the Cl--sensitive WNK1 kinase. Nature Communications
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WB/IB/PCA PubMed
de Weerd NA et al. (2017). A hot spot on interferon α/β receptor subunit 1 (IFNAR1) underpins its interaction with interferon-β and dictates signaling. J Biol Chem.
Applications
WB/IB/PCA PubMed
Shen et al. (2016). EMC6/TMEM93 suppresses glioblastoma proliferation by modulating autophagy. Cell Death & Disease
Applications
WB/IB/PCA PubMed
Chen et al. (2016). HBP1-mediated Regulation of p21 Protein through the Mdm2/p53 and TCF4/EZH2 Pathways and Its Impact on Cell Senescence and Tumorigenesis. Journal of Biological Chemistry
Applications
WB/IB/PCA PubMed
Xia D et al. (2016). MARCH2 regulates autophagy by promoting CFTR ubiquitination and degradation and PIK3CA-AKT-MTOR signaling. Autophagy.
Applications
WB/IB/PCA PubMed
Wang W et al. (2014). PIASxα ligase enhances SUMO1 modification of PTEN protein as a SUMO E3 ligase. J Biol Chem.
Applications
WB/IB/PCA PubMed
Wang Z et al. (2014). PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1. Autophagy.
Applications
IP/Co-IP PubMed
Li Y et al. (2013). A novel ER-localized transmembrane protein, EMC6, interacts with RAB5A and regulates cell autophagy. Autophagy.
Applications
WB/IB/PCA PubMed
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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