Hep-56.1D
Marke : CLS Cell Lines Service
Hep-56.1D Cells
General information
Description | Established from the primary hepatocellular carcinoma of C57BL/6J mice. |
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Organism | Mouse |
Tissue | Liver |
Disease | Hepatocellular carcinoma |
Synonyms | HEP-56.1D, 56.1D, 56.1d |
Characteristics
Age | Adult |
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Gender | Female |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | Hep-56.1D (Cytion catalog number 400204) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | Keratin 8, Keratin 18, Vimentin. |
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Tumorigenic | Yes, in C57BL/6J mice. In the third week, tumors will develop which are approx. 5-6 mm in diameter. |
Ploidy status | Aneuploid |
Mutational profile | p53mut, C:G -+ G:C transversion at codon 132 of mouse p53 exon 5, which corresponds to an amino acid change from cysteine to tryptophan. |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 25 to 30 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:8 is recommended |
Seeding density | 1 to 2 x 10^4 cells/cm^2 during routine culture |
Fluid renewal | Every 3 to 4 days |
Freezing recovery | >90% of the cells recovered from the freezing process within 24 to 48 h |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | M_18-3: 16 M_4-2: 20.3 M_6-7: 17 M_3-2: 14 M_19-2: 13 M_7-1: 26.2 M_1-1: 16 M_8-1: 16 M_2-1: 15 M_15-3: 22.3 M_6-4: 18 M_11-2: 16 M_1-2: 19 M_17-2: 15 M_12-1: 17 M_5-5: 17 M_X-1: 28 M_13-1: 17 Human D4/D8: - |