The Hepatitis A Virus (HAV) Antibody, IgG, BioAssay™ ELISA Kit is for the qualitative determination of human anti-hepatitis A virus (HAV) antibody (IgG) concentrations in serum.

Principle of the Assay:
This ELISA Kit employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HAV antigen. Samples are pipetted into the wells with mouse anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibody specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human anti-HAV antibody (IgG) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Specificity:
This assay has high sensitivity and excellent specificity for detection of human anti-HAV antibody (IgG). No significant crossreactivity or interference between human anti-HAV antibody (IgG) and analogues was observed.

Precision:
Intra-assay Precision: CV <15%
Inter-assay Precision: CV <15%

Kit Components:
227697A: Microtiter Strips, 1x48 wells or 1x96 wells. Pre-coated; ready to use.
227697B: Negative Control, 1x500ul or 1x1ml
227697C: Positive Control, 1x500ul or 1x1ml
227697D: HRP Conjugate, 1x6ml or 1x12ml
227697E: Wash Buffer, 20X, 1x30ml or 1x50ml
227697F: Sample Diluent, 1x6ml or 1x12ml
227697G: Substrate A, 1x3ml or 1x6ml
227697H: Substrate B, 1x3ml or 1x6ml:
227697K: Stop Solution, 1x3ml or 1x6ml

Storage and Stability:
Store kit at 4°C. The unopended kit is stable for 6 months after receipt. Once opened, the kit is stable up to 4 weeks at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.