His-tag Binding and Wash Buffer
Catalog Number: B2016383 (50 mL)
His-tag Binding and Wash Buffer is a high quality buffer solution used in affinity chromatography to purify proteins containing a polyhistidine (His-tag) sequence. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.
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Product Description
His-tag Binding and Wash Buffer
Catalog number: B2016383
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 mL
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: RT
Keywords: His Binding/Wash Buffer
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.
References:
1: Hefti MH, Milder FJ, Boeren S, Vervoort J, van Berkel WJ. A His-tag based immobilization method for the preparation and reconstitution of apoflavoproteins Biochim Biophys Acta. 2003 Jan 20;1619(2):139-43.
2: Al-Majdoub M, Opuni KF, Yefremova Y, Koy C, Lorenz P, El-Kased RF, Thiesen HJ, Glocker MO. A novel strategy for the rapid preparation and isolation of intact immune complexes from peptide mixtures J Mol Recognit. 2014 Sep;27(9):566-74.
3: Zhang Y, Wu L, Yu J, Mei J, Yi Y, Chen J, Ying G. Preparation of recombinant human thymic stromal lymphopoietin protein Prep Biochem Biotechnol. 2017 Oct 21;47(9):934-938.
4: de Marco A. Two-step metal affinity purification of double-tagged (NusA-His6) fusion proteins Nat Protoc. 2006;1(3):1538-43.
5: Guo F, Zhu G. Presence and removal of a contaminating NADH oxidation activity in recombinant maltose-binding protein fusion proteins expressed in Escherichia coli Biotechniques. 2012 Apr;52(4):247-53.
6: Keefe AD, Wilson DS, Seelig B, Szostak JW. One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag Protein Expr Purif. 2001 Dec;23(3):440-6.
7: Zhao JC, Zhao ZD, Wang W, Gao XM. Prokaryotic expression, refolding, and purification of fragment 450-650 of the spike protein of SARS-coronavirus Protein Expr Purif. 2005 Feb;39(2):169-74.
8: Fernandez-Rodriguez J, Marlovits TC. Induced heterodimerization and purification of two target proteins by a synthetic coiled-coil tag Protein Sci. 2012 Apr;21(4):511-9.
9: Sheikholvaezin A, Blomberg F, Ohrmalm C, Sjösten A, Blomberg J. Rational recombinant XMRV antigen preparation and bead coupling for multiplex serology in a suspension array Protein Expr Purif. 2011 Dec;80(2):176-84.
10: Liu F, Liao L, Chen J. Preparation of unglycosylated human caseinomacropeptide by engineering DAB Escherichia coli J Agric Food Chem. 2008 Feb 13;56(3):889-93.
Products Related to His-tag Binding and Wash Buffer can be found at Chemicals
Additional Information
| Weight | 2.6 oz |
|---|---|
| Dimensions | 3.3 × 1.2 × 1.2 in |