Semi-quantitative, sandwich-based, membrane cytokine array to detect 20 human angiogenesis protein biomarkers.
Human Angiogenesis Array C1
Human Angiogenesis Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 20 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Angiogenesis, Vascular Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| Angiogenin
| EGF
| ENA-78 (CXCL5)
| bFGF
| GRO alpha/beta/gamma
|
| IFN-gamma
| IGF-1
| IL-6
| IL-8 (CXCL8)
| Leptin
|
| MCP-1 (CCL2)
| PDGF-BB
| PLGF
| RANTES (CCL5)
| TGF beta 1
|
| TIMP-1
| TIMP-2
| Thrombopoietin (TPO)
| VEGF-A
| VEGF-D
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
- Human Angiogenesis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Antagonistic SMAD2/3 control of TIMP-1, VEGF-A, and hypoxia signaling in myofibroblasts shapes histotype-specific angiogenesis in lung cancer
- Species:
- Human
- Sample Type:
- CM from TAFs cells
- Year:
- 2026
- Journal Name:
- Cell Death & Disease
- PubMed ID:
- 41912498
The Algal Antioxidant Carotenoid Diatoxanthin as a Modulator of Inflammation and Angiogenesis in Triple-Negative Breast Cancer Cells
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (secretome of BT-549 cells)
- Year:
- 2026
- Journal Name:
- Antioxidants
- PubMed ID:
- 41750586
Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (CM and CM + ADSC tissue sheets)
- Year:
- 2024
- Journal Name:
- Bioactive Materials
- PubMed ID:
- 38689657
Platelet-rich plasma promotes cellular recovery from nicotine-induced toxicity via autophagy modulation
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned medium from GF)
- Year:
- 2026
- Journal Name:
- Scientific Reports
- PubMed ID:
- 41663725
Obtention of conditional media from Wharton’s gelatin mesenchymal stromal cells in a dynamic culture system
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (medio condicionado conditioned media of Wharton's jelly mesenchymal stromal…)
- Year:
- 2026
- Journal Name:
- Biomedica : revista del Instituto Nacional de Salud
- PubMed ID:
- 41875459
Anti-TP0136 Antibody Delayed Syphilis Wound Healing by Inhibiting Angiogenesis Through the VEGF-A Signaling Pathway: A Novel View in Nonhealing Syphilis Wounds
- Year:
- 2026
- Journal Name:
- FASEB Journal
- PubMed ID:
- 41524220
Human platelet lysate-cultured adipose-derived stem cell sheets promote angiogenesis and accelerate wound healing via CCL5 modulation
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned medium of ASC sheets)
- Year:
- 2024
- Journal Name:
- Stem Cell Research & Therapy
- PubMed ID:
- 38853252
The role of IGF-2 and its variants in enhancing endothelial migration and angiogenesis
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned medium CM from HMEC-1)
- Year:
- 2025
- Journal Name:
- Frontiers in Cell and Developmental Biology
- PubMed ID:
- 40454313
Exploring association of melanoma-specific Bcl-xL with tumor immune microenvironment
- Species:
- Human
- Sample Type:
- M14 cell
- Year:
- 2023
- Journal Name:
- Journal of Experimental & Clinical Cancer Research
Studying sprouting angiogenesis under combination of oxygen gradients and co-culture of fibroblasts using microfluidic cell culture model
- Species:
- Human
- Sample Type:
- human umbilical vein endothelial cells (HUVECs) , MRC-5 cell
- Year:
- 2023
- Journal Name:
- Materials Today Bio