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> Human Epinephrine/Adrenaline,EPI ELISA Kit

Human Epinephrine/Adrenaline,EPI ELISA Kit

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Katalog-Nummer CSB-E08677h-96T

Size : 96T

Marke : Cusabio


CSB-E08677h
Size 96T,5×96T,10×96T

Product Details

Target Name
Epinephrine/Adrenaline,EPI
Abbreviation
EPI
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
2 pg/mL-400 pg/mL
Sensitivity
1 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 92  
Range % 87-98  
1:2 Average % 95  
Range % 90-99  
1:4 Average % 90  
Range % 85-96  
1:8 Average % 90  
Range % 84-98  
Recovery
The recovery of human EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 80-94  
EDTA plasma (n=4) 98 92-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
400 0.213 0.210 0.212    
100 0.408 0.391 0.400    
32 0.613 0.631 0.622    
8 1.062 1.003 1.033    
2 1.593 1.516 1.555    
0 2.467 2.412 2.440    
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

The human Epinephrine/Adrenaline (EPI) ELISA kit provides a means for quantitatively measuring Epinephrine levels in human serum, plasma, and tissue homogenates. With a detection range of 2 pg/mL to 400 pg/mL and a sensitivity of 1 pg/mL, this kit allows for precise and accurate measurements of Epinephrine levels. This content looks too robotic. The assay principle is competitive, and the detection wavelength is set at 450 nm, ensuring reliable and reproducible results. The assay time ranges from 1 to 5 hours, making it efficient for laboratory use, and requires a sample volume of 50-100 µl...

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