- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-35 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled IL-35 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
Human Interleukin 35(IL-35) ELISA Kit
Katalog-Nummer CSB-E13126h-5x96T
Size : 5x96T
Marke : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Interleukin 35(IL-35)
Abbreviation
IL-35
Species
Homo sapiens (Human)
Sample Types
serum, cell culture supernates, urine, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-103 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 90-106 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 88-109 | |||||
Recovery
| The recovery of human IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Materials provided
Materials not provided
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
The CUSABIO Human Interleukin 35 (IL-35) ELISA kit is specifically tailored for detecting IL-35 in serum, cell culture supernates, urine, and tissue homogenates from humans. With a detection range of 62.5 pg/mL to 4000 pg/mL and a sensitivity of 15.6 pg/mL, this assay offers a reliable method for quantitatively assessing IL-35 levels. The assay principle is based on a sandwich ELISA method, ensuring accurate and precise measurements within a sample volume of 50-100 µl. The detection wavelength is set at 450 nm, and the assay can be completed within 1-5 hours. Notably, this ELISA kit has been...
Citations
- JY Cheng, HH Tsai, JT Hung, TH Hung,Advanced Science,2025
- M Ma,Oncoimmunology,2024
- R Wang,Central European Journal of Immunology,2023
- C Qiu,International immunopharmacology,2023
- Z Li,Thoracic Cancer,2022
- H Jiang,Pathogens and Disease,2021
- A Vultaggio,Scientific reports,2021
- Q Xie,International Immunopharmacology,2020
- J Zhang,Clinical Reviews in Allergy & Immunology,2020
- W Li,Respir. Res,2020
Customer Reviews and Q&A
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■ Q&A
Q:
I am interested to know more about the IL-35 ELISA Kit (CSB-E13126h). Is the capture antibody the same as the detection antibody?
A:
Thanks for your inquiry.
IL35 is a dimer complex composed of 2 subunits EBI3 and p35.
The kit uses 2 antibodies as below, which are designed for different subunits.
Coated anti: Mouse monoclonal antibody
Detection anti: Goat polyclonal antibody Pls let me know if you have any further questions. Thank you.
IL35 is a dimer complex composed of 2 subunits EBI3 and p35.
The kit uses 2 antibodies as below, which are designed for different subunits.
Coated anti: Mouse monoclonal antibody
Detection anti: Goat polyclonal antibody Pls let me know if you have any further questions. Thank you.
Q:
I am planning to carry out a study to assess level of Interleukin from Gingival Crevicular Fluid in Chronic Periodontitis patients before and after treatment.Do you have the Interleukin 35? How many sample can be analysed?
A:
Thanks for your inquiry. We have this kit CSB-E13126h,Human Interleukin 35(IL-35) ELISA Kit to provide.This kit isapplied with sandwich enzyme immunoassay technique.For a 96T sandwich ELISA kit (doubleantibody), we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for
the sample, and in this way you can test 40...
the sample, and in this way you can test 40...
Q:
I had a question about the specificity of the human IL-35 ELISA kit, CSB-E13126h. Is the detection antibody specifically detecting IL-35 or is detecting the subunits of IL-35 (EBI-3 & IL-12p35)?
A:
This kit CSB-E13126h detects IL35 compound. The two antibodies in the kit are designed specifically for two subunits.