RayBio® Human MMP-13 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human MMP-13 ELISA Kit Standard Curve
Human MMP-13 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 6 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 6 pg/ml - 6000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested Human Angiopoietin-1, Angiostatin , B7-1, BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, E-Selectin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, M-CSF R, MMP-1, 2, 3, 7, 8, 9, 10 and 12, PDGF-AB, SDF-1b, Tie-1, Tie-2, TIMP-3. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P45452 |
| Gene Id | 4322 |
| Gene Symbols | MMP13 |
| Protein Name / Synonyms | Collagenase 3 (EC 3.4.24.-) (Matrix metalloproteinase-13) (MMP-13) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human MMP-13 qIPCR ELISA Kit
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human MMP-13 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified MMP-13 (MMP13), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Electroacupuncture Ameliorates Knee Osteoarthritis By Rebalancing T Cell Homeostasis as Revealed By Immune Repertoire (IR) Sequencing
- Species:
- Human
- Sample Type:
- Serum (KOA patients, baseline and post-treatment)
- Year:
- 2025
- Journal Name:
- Combinatorial chemistry & high throughput screening
- PubMed ID:
- 39136509
IL-40 is up-regulated in the synovial fluid and cartilage of osteoarthritis patients and contributes to the alteration of chondrocytes phenotype in vitro
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (NHAC-kn chondrocytes + IL-40)
- Year:
- 2024
- Journal Name:
- Arthritis Research & Therapy
- PubMed ID:
- 39080724
Chemically modified small interfering RNA targeting Hedgehog signaling pathway for rheumatoid arthritis therapy
- Species:
- Human
- Sample Type:
- fibroblast-like synoviocytes (FLSs)
- Year:
- 2022
- Journal Name:
- Molecular Therapy-Nucleic Acids
Simvastatin and fluvastatin attenuate trauma-induced cell death and catabolism in human cartilage
- Species:
- Human
- Sample Type:
- cartilage tissue
- Year:
- 2022
- Journal Name:
- Frontiers in bioengineering and biotechnology
- PubMed ID:
- 36159664
In Vitro Study of Licorice on IL-1β-Induced Chondrocytes and In Silico Approach for Osteoarthritis
- Species:
- Human
- Sample Type:
- Other / Unspecified (media of SW1353 cells)
- Year:
- 2021
- Journal Name:
- Pharmaceuticals
- PubMed ID:
- 34959737
Toll-like receptor involvement in adolescent scoliotic facet joint degeneration
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (explant culture media); Plant / Food / Environmental
- Year:
- 2020
- Journal Name:
- Journal of Cellular and Molecular Medicine
- PubMed ID:
- 32853438
Identification of Differential Expression Cytokines in Hemolysis, Elevated Liver Enzymes, and Low Platelet Syndrome by Proteome Microarray Analysis and Further Verification
- Species:
- Human
- Sample Type:
- Serum (21 HELLP patients, 21 controls)
- Year:
- 2021
- Journal Name:
- Cell Transplantation
- PubMed ID:
- 33757334
Effect of Electro-Acupuncture (EA) and Manual Acupuncture (MA) on Markers of Inflammation in Knee Osteoarthritis
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- Journal of pain research
Baicalein ameliorates inflammatory-related apoptotic and catabolic phenotypes in human chondrocytes
- Species:
- Human
- Sample Type:
- Conditioned Media (Human articular chondrocytes cell)
- Year:
- 2014
- Journal Name:
- International Immunopharmacology
Curcumin and o-Vanillin Exhibit Evidence of Senolytic Activity in Human IVD Cells In Vitro
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (pooled cell pellet culture media)
- Year:
- 2019
- Journal Name:
- Journal of Clinical Medicine
- PubMed ID:
- 30934902