Human TNF Alpha Protein (Recombinant)

Recombinant Protein

TNF Alpha

Human

Unmodified

17.3 kDa

E. coli

E. coli

Greater than 95% by SDS-PAGE

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective

The ED50 as determined in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D is less than 0.1 ng/ml.

Less than 1.0 EU/µg protein (determined by LAL method).

Lyophilized from 0.2 µm filtered 20 mM PBs, pH 7.0, 150 mM NaCl

Store at -80°C for up to 1 year. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

Uniprot: P01375 NCBI: NM_000594 NP_000585.2