Origin:

This cell line was derived from L-M(TK-) cells that were transfected with a Wnt-3A expression vector and stable clones were selected in medium containing G418.

Species:

Mus musculus, mouse

Tissue:

Subcutaneous connective tissue; areolar and adipose

Morphology:

Fibroblast

Properties:

Adherent

Host:

Male, strain C3H/An

Medium:

AddexBio-formulated DMEM (C0003-01), or equivalent medium + 0.4 mg/mL G-418 + 10% FBS (Wnt-3A Conditioned Medium protocol please refer to datasheet)

Subculture:

When 80% confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:10; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37°C. Wnt-3A Conditioned Medium protocol please refer to datasheet.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO